BRENDA - Enzyme Database show
show all sequences of 6.3.3.4

New reactions in clavulanic acid biosynthesis

Townsend, C.A.; Curr. Opin. Chem. Biol. 6, 583-589 (2002)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54500
-
x * 54500, deduced from gene sequence
Streptomyces clavuligerus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N2-(carboxyethyl)-L-arginine + ATP
Streptomyces clavuligerus
overview on biological role
AMP + diphosphate + deoxyamidinoproclavaminate
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces clavuligerus
P0DJQ7
-
-
Reaction
Reaction
Commentary
Organism
ATP + L-N2-(2-carboxyethyl)arginine = AMP + diphosphate + deoxyamidinoproclavaminate
overview
Streptomyces clavuligerus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N2-(carboxyethyl)-L-arginine + ATP
overview on biological role
639036
Streptomyces clavuligerus
AMP + diphosphate + deoxyamidinoproclavaminate
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 54500, deduced from gene sequence
Streptomyces clavuligerus
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
ATP-binding site
Streptomyces clavuligerus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
ATP-binding site
Streptomyces clavuligerus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54500
-
x * 54500, deduced from gene sequence
Streptomyces clavuligerus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N2-(carboxyethyl)-L-arginine + ATP
Streptomyces clavuligerus
overview on biological role
AMP + diphosphate + deoxyamidinoproclavaminate
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N2-(carboxyethyl)-L-arginine + ATP
overview on biological role
639036
Streptomyces clavuligerus
AMP + diphosphate + deoxyamidinoproclavaminate
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 54500, deduced from gene sequence
Streptomyces clavuligerus
Other publictions for EC 6.3.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742159
Shrestha
-
Heterologous production of cl ...
Streptomyces clavuligerus, Streptomyces clavuligerus DSM 738
Biotechnol. Bioprocess Eng.
22
359-365
2017
-
1
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
728238
Labonte
Engineering the synthetic pote ...
Streptomyces clavuligerus
MedChemComm
3
960-966
2012
-
-
-
-
3
-
-
13
-
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
13
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
13
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
639033
Miller
The catalytic cycle of beta-la ...
Streptomyces clavuligerus
Proc. Natl. Acad. Sci. USA
99
14752-14757
2002
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639036
Townsend
New reactions in clavulanic ac ...
Streptomyces clavuligerus
Curr. Opin. Chem. Biol.
6
583-589
2002
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639034
Miller
Structure of beta-lactam synth ...
Streptomyces clavuligerus
Nat. Struct. Biol.
8
684-689
2001
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639032
Bachmann
Kinetic mechanism of the beta- ...
Streptomyces clavuligerus
Biochemistry
39
11187-11193
2000
-
-
-
-
-
-
3
2
-
-
-
1
-
1
-
-
1
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
-
1
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639031
Bachmann
beta-Lactam synthetase: a new ...
Streptomyces clavuligerus
Proc. Natl. Acad. Sci. USA
95
9082-9086
1998
-
-
1
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639035
McNaughton
-
beta-Lactam synthetase: implic ...
Streptomyces clavuligerus
Chem. Commun. (Camb.)
1998
2325-2326
1998
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-