Literature summary for 6.3.2.8 extracted from

Videau, P.; Rivers, O.S.; Ushijima, B.; Oshiro, R.T.; Kim, M.J.; Philmus, B.; Cozy, L.M.
Mutation of the murC and murB genes impairs heterocyst differentiation in Anabaena sp. strain PCC 7120 (2016), J. Bacteriol., 198, 1196-1206 .

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Protein Variants

Protein Variants	Comment	Organism
additional information	screening and identification of a transposon mutant impaired in diazotrophic growth, the mutant strain contains a transposon insertion at nucleotide position -476 relative to the annotated translational start site of gene alr5065. The mutant, designated C6, is unable to grow diazotrophically and displays a deficit in heterocyst differentiation, producing 4.5% heterocysts compared to the 9% produced by the wild type. Proper heterocyst production is restored in mutant C6 when the mutation is complemented with both alr5065 and alr5066, encoding enzymes MurC and MurB, as PpetE-murC/B (pPJAV328) in the presence of copper, but not when either gene is introduced individually, which suggests that alr5065 and alr5066 may be in an operon and the transposon insertion affectes the expression of both genes. After repeated rounds of subculturing, C6 loses viability and ceases to grow in culture. Generation of murC mutant strain UHM351, in which murC is inactivated by pPJAV309, phenotype, overview	Nostoc sp. PCC 7120 = FACHB-418

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Nostoc sp. PCC 7120 = FACHB-418

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine	Nostoc sp. PCC 7120 = FACHB-418	-	ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine	-	?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine	Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576	-	ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine	-	?

Organism

Organism	UniProt	Comment	Textmining
Nostoc sp. PCC 7120 = FACHB-418	Q8YM75	-	-
Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576	Q8YM75	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine	-	Nostoc sp. PCC 7120 = FACHB-418	ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine	-	?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine	-	Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576	ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine	-	?

Synonyms

Synonyms	Comment	Organism
alr5065	-	Nostoc sp. PCC 7120 = FACHB-418
MurC	-	Nostoc sp. PCC 7120 = FACHB-418
UDP-N-acetylmuramate:L-alanine ligase	-	Nostoc sp. PCC 7120 = FACHB-418

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Nostoc sp. PCC 7120 = FACHB-418

General Information

General Information	Comment	Organism
malfunction	mutation of the gene murC impairs heterocyst differentiation in Nostoc sp. PCC 7120. Gene murC depletion leads to aberrant expression of patS in all cells of the filament	Nostoc sp. PCC 7120 = FACHB-418
metabolism	enzyme MurC catalyzes the third step in peptidoglycan biosynthesis	Nostoc sp. PCC 7120 = FACHB-418
physiological function	the enzyme is important in peptidoglycan biosynthesis. Heterocyst development has been shown to require proper peptidoglycan remodeling. Determination of a direct link between peptidoglycan synthesis and the maintenance of a biological pattern in a multicellular organism. Nostoc sp. PCC 7120 differentiates a periodic pattern of specialized heterocyst cells, requiring peptidoglycan synthesis by the murine ligase genes murC (alr5065) and murB (alr5066) for maintenance of patterned gene expression, filament integrity, and overall development, overview	Nostoc sp. PCC 7120 = FACHB-418

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Release 2023.1 (January 2023)