Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli with an N-terminal His6tag | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + (1R,2S,6R)-anticapsin | Bacillus subtilis | the enzyme is involved in the biosynthesis of the dipeptide antibiotic bacilysin | ADP + phosphate + bacilysin | i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | ? | |
ATP + L-alanine + (1R,2S,6R)-anticapsin | Bacillus subtilis 168 | the enzyme is involved in the biosynthesis of the dipeptide antibiotic bacilysin | ADP + phosphate + bacilysin | i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P39641 | - |
- |
Bacillus subtilis 168 | P39641 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + (1R,2S,5R,6S)-dihydroanticapsin | - |
Bacillus subtilis | ADP + phosphate + dihydrobacilysin | - |
? | |
ATP + L-alanine + (1R,2S,5R,6S)-dihydroanticapsin | - |
Bacillus subtilis 168 | ADP + phosphate + dihydrobacilysin | - |
? | |
ATP + L-alanine + (1R,2S,6R)-anticapsin | the enzyme is involved in the biosynthesis of the dipeptide antibiotic bacilysin | Bacillus subtilis | ADP + phosphate + bacilysin | i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | ? | |
ATP + L-alanine + (1R,2S,6R)-anticapsin | when the DELTAbacC extract is exposed to the enzyme (BacD) and the dihydroanticapsin:NAD+ 7-oxidoreductase (BacC) the C7-hydroxyl of hydroanticapsin is oxidized to generate anticapsin. (1R,2S,6R)-anticapsin i.e. 3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | Bacillus subtilis | ADP + phosphate + bacilysin | i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | ? | |
ATP + L-alanine + (1R,2S,6R)-anticapsin | the enzyme is involved in the biosynthesis of the dipeptide antibiotic bacilysin | Bacillus subtilis 168 | ADP + phosphate + bacilysin | i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | ? | |
ATP + L-alanine + (1R,2S,6R)-anticapsin | when the DELTAbacC extract is exposed to the enzyme (BacD) and the dihydroanticapsin:NAD+ 7-oxidoreductase (BacC) the C7-hydroxyl of hydroanticapsin is oxidized to generate anticapsin. (1R,2S,6R)-anticapsin i.e. 3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | Bacillus subtilis 168 | ADP + phosphate + bacilysin | i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | ? | |
ATP + L-alanine + 3-[(1S)-4-oxocyclohex-2-en-1-yl]-L-alanine | 3-[(1S)-4-oxocyclohex-2-en-1-yl]-L-alanine is a surrogate substrate for the enzyme, because currently there is no route for purifying dihydroanticapsin (with the epoxide intact) from DELTAbacC strain extracts. A simultaneous incubation of the enzyme (ligase BacD) with oxidoreductase BaC, (2S,4S,7R)-tetrahydrotyrosine, NAD+, L-Ala, and ATP yields the dipeptide product L-Ala-2S,4S-cyclohexenoyl-Ala. This result indicates the BacD ligase can intercept the nascent 4S-cyclohexenonyl-Ala and ligate its N-terminus to L-Ala before its free amino group undergoes intramolecular addition | Bacillus subtilis | ADP + phosphate + L-alanyl-[3-[(1S)-4-oxocyclohex-2-en-1-yl]-L-alanine] | - |
? | |
ATP + L-alanine + 3-[(1S)-4-oxocyclohex-2-en-1-yl]-L-alanine | 3-[(1S)-4-oxocyclohex-2-en-1-yl]-L-alanine is a surrogate substrate for the enzyme, because currently there is no route for purifying dihydroanticapsin (with the epoxide intact) from DELTAbacC strain extracts. A simultaneous incubation of the enzyme (ligase BacD) with oxidoreductase BaC, (2S,4S,7R)-tetrahydrotyrosine, NAD+, L-Ala, and ATP yields the dipeptide product L-Ala-2S,4S-cyclohexenoyl-Ala. This result indicates the BacD ligase can intercept the nascent 4S-cyclohexenonyl-Ala and ligate its N-terminus to L-Ala before its free amino group undergoes intramolecular addition | Bacillus subtilis 168 | ADP + phosphate + L-alanyl-[3-[(1S)-4-oxocyclohex-2-en-1-yl]-L-alanine] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alanine-anticapsin ligase | - |
Bacillus subtilis |
BacD | - |
Bacillus subtilis |
BSU37710 | locus name | Bacillus subtilis |
L-Ala-L-aniticapsin ligase | - |
Bacillus subtilis |
L-alanine-L-aniticapsin ligase | - |
Bacillus subtilis |
YwfE | - |
Bacillus subtilis |