Cloned (Comment) | Organism |
---|---|
- |
Streptococcus agalactiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-buthionine-S-sulfoximine | competitive with L-glutamate | Streptococcus agalactiae | |
additional information | no significant inhibition by cystamine, L-methionine-SR-sulfoximine and GSH | Streptococcus agalactiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.156 | - |
L-cysteine | - |
Streptococcus agalactiae | |
22 | - |
L-glutamate | - |
Streptococcus agalactiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities, gel filtration | Streptococcus agalactiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus agalactiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His6-gamma-GCS-GS, bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities | Streptococcus agalactiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.3 | - |
- |
Streptococcus agalactiae |
Storage Stability | Organism |
---|---|
4°C, -20°C or -80°C, the isolated enzyme loses activity quickly (about 50% in 24 h), with or without glycerol | Streptococcus agalactiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + DL-alpha-aminoadipate + L-cysteine | about 10% of the activity with L-glutamate | Streptococcus agalactiae | ADP + phosphate + DL-aminoadipyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | - |
Streptococcus agalactiae | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities | Streptococcus agalactiae |
Synonyms | Comment | Organism |
---|---|---|
gamma-GCS-GS | bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities | Streptococcus agalactiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.9 | - |
L-buthionine-S-sulfoximine | - |
Streptococcus agalactiae |