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Literature summary for 6.2.1.12 extracted from

  • Lee, Y.; Jeon, Y.; Lee, J.S.; Kim, B.; Lee, C.H.; Ahn, J.
    Enzymatic synthesis of phenolic CoAs using 4-coumarate:coenzyme A ligase (4CL) from rice (2007), Bull. Korean Chem. Soc., 28, 365-366.
No PubMed abstract available
Search for more literature for 6.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
gene R4CL, expression in Escherichia coli strain BL21 Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Oryza sativa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 x * 86000, recombinant enzyme, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Oryza sativa
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
additional information Oryza sativa the enzyme catalyzes the conversion of 4-coumaric acid into coumaroyl-CoA and a few related substrates into their corresponding products such as cinnamoyl-CoA, caffeoyl-CoA, and feruloyl-CoA in a process utilizing ATP, and thus channels the common, phenylalanine-derived building block into the widely distinct branches of general phenylpropanoid metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Q6ETN3 gene R4CL, putative 4-coumarate coenzyme A ligase, i.e. Os02g0177600 protein; gene R4CL
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21 to near homogeneity Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA
-
 Oryza sativa AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
additional information the enzyme catalyzes the conversion of 4-coumaric acid into coumaroyl-CoA and a few related substrates into their corresponding products such as cinnamoyl-CoA, caffeoyl-CoA, and feruloyl-CoA in a process utilizing ATP, and thus channels the common, phenylalanine-derived building block into the widely distinct branches of general phenylpropanoid metabolism Oryza sativa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 86000, recombinant enzyme, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
4-coumarate coenzyme A ligase
-
 Oryza sativa
4-Coumarate:coenzyme A ligase
-
 Oryza sativa

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Oryza sativa