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Literature summary for 6.2.1.12 extracted from

  • Hamada, K.; Nishida, T.; Yamauchi, K.; Fukushima, K.; Kondo, R.; Tsutsumi, Y.
    4-Coumarate:coenzyme A ligase in black locust (Robinia pseudoacacia) catalyses the conversion of sinapate to sinapoyl-CoA (2004), J. Plant Res., 117, 303-310.
    View publication on PubMed
Search for more literature for 6.2.1.12

Inhibitors

Inhibitors Comment Organism Structure
additional information the sinapate-converting activity is not inhibited by other cinnamate derivatives, such as p-coumarate, caffeate or ferulate Robinia pseudoacacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, SDS-PAGE Robinia pseudoacacia

Organism

Organism UniProt Comment Textmining
Robinia pseudoacacia
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
shoot
-
 Robinia pseudoacacia
-
xylem 4CL2 is the major isoform in developing xylem extract, 4CL3 is a very minor one Robinia pseudoacacia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA isoenzyme 4CL1 exhibits very high activity towards p-coumarate, isoenzymes 4CL2 and 4CL3 are active Robinia pseudoacacia AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 5-hydroxyferulate + CoA isoenzyme 4CL2, weak activity, isoenzyme 4CL1 and 4CL3 show no activity Robinia pseudoacacia AMP + diphosphate + 5-hydroxyferuloyl-CoA
-
 ?
ATP + caffeate + CoA isoenzyme 4CL1, 4CL2 and 4CL3 show activity Robinia pseudoacacia AMP + diphosphate + caffeoyl-CoA
-
 ?
ATP + ferulate + CoA isoenzyme 4CL2 and 4CL3 are active, no activity with isoenzyme 4CL1 Robinia pseudoacacia AMP + diphosphate + feruloyl-CoA
-
 ?
ATP + sinapate + CoA isoenzymes 4CL2 and 4CL3 are active, isoenzyme 4CL1 is inactive Robinia pseudoacacia AMP + diphosphate + sinapoyl-CoA
-
 ?
additional information the isoform 4CL1 has a strong substrate preference for p-coumarate, but lacked the activity for ferulate and sinapate. The other hand, 4CL2 and 4CL3 display activity towards sinapate and also possess high activity towards caffeate as well as p-coumarate Robinia pseudoacacia ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE Robinia pseudoacacia

Synonyms

Synonyms Comment Organism
4-coumarate:coenzyme A (CoA) ligase
-
 Robinia pseudoacacia
4CL
-
 Robinia pseudoacacia