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Literature summary for 6.2.1.1 extracted from
- Enzymatic characterization of two acetyl-CoA synthetase genes from Populus trichocarpa (2016), SpringerPlus, 5, 818 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Populus trichocarpa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
Sodium acetate | isoform ACS1, at pH 7.8 and 25°C | Populus trichocarpa |  |
| 0.72 | - |
Sodium acetate | isoform ACS2, at pH 7.8 and 25°C | Populus trichocarpa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate + CoA | Populus trichocarpa | - |
AMP + diphosphate + acetyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus trichocarpa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Populus trichocarpa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | highest expression | Populus trichocarpa | - |
| phloem | - |
Populus trichocarpa | - |
| root | - |
Populus trichocarpa | - |
| xylem | - |
Populus trichocarpa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate + CoA | - |
Populus trichocarpa | AMP + diphosphate + acetyl-CoA | - |
? | |
| ATP + sodium acetate + CoA | - |
Populus trichocarpa | AMP + acetyl-CoA + sodium diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, isoform ACS2, SDS-PAGE | Populus trichocarpa |
| ? | x * 60000, isoform ACS1, SDS-PAGE | Populus trichocarpa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Acetyl-CoA synthase | - |
Populus trichocarpa |
| ACS1 | isoform | Populus trichocarpa |
| ACS2 | isoform | Populus trichocarpa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Populus trichocarpa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 55 | the enzyme activity is more than 90% of maximum over a broad temperature range between 25 and 40°C, and both recombinant isoform proteins retain more than 60% of their maximum enzymatic activity at temperatures between 15 and 45°C. Isoform ACS1 enzyme activity decreases to 12% of its maximum at 55°C | Populus trichocarpa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
isoform ACS1 | Populus trichocarpa |
| 8 | - |
isoform ACS2 | Populus trichocarpa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | isoform ACS1 shows high levels of activity in a pH range of 7.0-8.0. Isoform ACS2 shows high levels of activity in a pH range of 7.5-8.5. Little activity is detected for either isoform ACS1 or ACS2 at pH levels below 5.0 or above 9.0 | Populus trichocarpa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Populus trichocarpa | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Populus trichocarpa | isoform ACS2, isoelectric focusing | - |
8.7 |
| Populus trichocarpa | isoform ACS1, isoelectric focusing | - |
8.8 |
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