Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanothermobacter thermautotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
A498A | alteration does not significantly affect the Km for any substrate but reduces the turnover rate kcat 41fold | Methanothermobacter thermautotrophicus |
A500T | kcat value decreases 44fold | Methanothermobacter thermautotrophicus |
D502A | inactive mutant enzyme | Methanothermobacter thermautotrophicus |
D502E | inactive mutant enzyme | Methanothermobacter thermautotrophicus |
D502N | inactive mutant enzyme | Methanothermobacter thermautotrophicus |
G501A | two- to threefold reduced Km values for acetate, ATP and CoA. The turnover rate is over 200fold reduced | Methanothermobacter thermautotrophicus |
T499A | kcat value decreases 83fold | Methanothermobacter thermautotrophicus |
Y498F | inactive mutant enzyme | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
CoA | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
0.1 | - |
CoA | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus | |
0.19 | - |
CoA | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
0.24 | - |
CoA | pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
0.5 | 1 | CoA | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
1.4 | - |
acetate | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
1.7 | - |
ATP | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
1.7 | - |
ATP | pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
1.7 | - |
ATP | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus | |
2.1 | - |
acetate | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
3 | - |
acetate | pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
3.3 | - |
ATP | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
3.5 | - |
acetate | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
3.6 | - |
ATP | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
7.5 | - |
acetate | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | Q2I0L7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | important role for motif III (498YTAGD502) in catalysis. The highly conserved Tyr in the first position may play a key role in active-site architecture through interaction with a highly conserved active-site Gln. The invariant Asp in the fifth position plays a critical role in ATP binding and catalysis through interaction with the 2'- and 3'-OH groups of the ribose moiety of ATP | Methanothermobacter thermautotrophicus | AMP + diphosphate + acetyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACS1 | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ATP | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
0.3 | - |
CoA | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
0.3 | - |
acetate | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
0.8 | - |
ATP | pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
0.8 | - |
acetate | pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
0.8 | - |
CoA | pH pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
1.5 | - |
ATP | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
1.5 | - |
CoA | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
1.5 | - |
acetate | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
1.6 | - |
ATP | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus | |
1.6 | - |
CoA | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus | |
1.6 | - |
acetate | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus | |
66.6 | - |
ATP | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
66.6 | - |
CoA | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
66.6 | - |
acetate | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | assay at | Methanothermobacter thermautotrophicus |