Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermine | stimulates only at low Mg2+ concentrations | Bombyx mori |
General Stability | Organism |
---|---|
sensitive to repeated freezing and thawing | Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00063 | - |
liver tRNA | Bombyx mori tRNA | Bombyx mori | |
0.34 | - |
alanine | - |
Bombyx mori | |
1.7 | - |
ATP | - |
Bombyx mori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimum: 5-10 mM | Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
1 * 110000, SDS-PAGE | Bombyx mori |
151000 | - |
gel filtration | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alanine + tRNAAla | Bombyx mori | key role in protein biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
silk gland | posterior | Bombyx mori | - |
Storage Stability | Organism |
---|---|
-80°C, 3 months, retains approximately 90% of activity | Bombyx mori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alanine + tRNAAla | key role in protein biosynthesis | Bombyx mori | ? | - |
? | |
ATP + L-alanine + liver tRNA | - |
Bombyx mori | ? | - |
? | |
ATP + L-alanine + tRNAAla | - |
Bombyx mori | AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 110000, SDS-PAGE | Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
alanyl-tRNA synthesis | Bombyx mori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 7.5 | 6.4: about 60% of maximal activity, 7.5: optimum | Bombyx mori |