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Literature summary for 6.1.1.20 extracted from

  • Moghal, A.; Hwang, L.; Faull, K.; Ibba, M.
    Multiple quality control pathways limit non-protein amino acid use by yeast cytoplasmic phenylalanyl-tRNA synthetase (2016), J. Biol. Chem., 291, 15796-15805 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0423
-
 L-phenylalanine at pH 7.5 and 37°C Saccharomyces cerevisiae
0.3107
-
 2-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae
1.15
-
 3-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe Saccharomyces cerevisiae
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-L-tyrosine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + 2-L-tyrosyl-tRNAPhe
-
 ?
ATP + 3-L-tyrosine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + 3-L-tyrosyl-tRNAPhe
-
 ?
ATP + L-alanine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + L-alanyl-tRNAPhe
-
 ?
ATP + L-leucine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + L-leucyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
additional information very low activity with L-DOPA and 4-L-tyrosine Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
Phenylalanine-tRNA synthetase
-
 Saccharomyces cerevisiae
PheRS
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26
-
 3-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae
39.4
-
 2-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae
58.2
-
 L-phenylalanine at pH 7.5 and 37°C Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
23
-
 3-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae
130
-
 2-L-tyrosine at pH 7.5 and 37°C Saccharomyces cerevisiae
1400
-
 L-phenylalanine at pH 7.5 and 37°C Saccharomyces cerevisiae