Cloned (Comment) | Organism |
---|---|
the two subunits of hcPheRS are cloned into two different plasmids, the alpha subunit, residues 1-508, into the vector pET21b+, the beta-subunit, residues 1-589, into pET28b+, for expression in Escherichia coli BLRDE3 cells, no affinity tag is used | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
the structure of human cytoplasmic phenylalanyl-tRNA synthetase is determined to a resolution of 3.3 A | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
247000 | - |
heterotetramer | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O95363 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation is followed by four chromatography columns, anion exchange on a DEAE-Sepharose column, a heparin-Sepharose column, concentrating the protein on a TSK hydrophobic interaction column and finally gel filtration on a column of superfine Sephadex G-200 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | - |
Homo sapiens | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | two alpha and two beta subunits | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
cytoplasmic phenylalanyl-tRNA synthetase | - |
Homo sapiens |
cytosolic phenylalanyl-tRNA synthetase | - |
Homo sapiens |
hcPheRS | - |
Homo sapiens |
Phenylalanyl-tRNA synthetase | - |
Homo sapiens |
PheRS | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |