Activating Compound | Comment | Organism | Structure |
---|---|---|---|
heme | enhances activity | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | human mini, but not full-length, TrpRS has angiostatic activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cDNA fragments of human full-length TrpRS, aa 1-471, mini TrpRS, aa 48-471, and T1 TrpRS, aa 71-471, are separately cloned into the pET20b vector for expression in Escherichia coli BL21DE3 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H130R | full-length H130R TrpRS is constitutively active, the mini H130R TrpRS mutant forms disulfide bonds and there are additional functional differences between the full-length and mini forms | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | enhances activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Homo sapiens | - |
AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P23381 | - |
- |
Purification (Comment) | Organism |
---|---|
the recombinant six-His-tagged proteins are purified on a nickel affinity column | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Homo sapiens | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mini tryptophanyl-tRNA synthetase | truncated form | Homo sapiens |
TrpRS | - |
Homo sapiens |
Tryptophanyl-tRNA synthetase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
aminoacylation assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
aminoacylation assay | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |