Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and anaylsis, genetic structure, aaRS domain organization and sequence conservation and the sense/antisense coding hypothesis, overview, expression of FLAG-tagged wild-type and mutant enzymes and isolated catalytic domains in Escherichia coli strain BL21(DE3) inclusion bodies | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
D146A | site-directed mutagensis, the mutant shows highly reduced activity compared to the wild-type enzyme | Geobacillus stearothermophilus |
additional information | construction of a catalytically active wild-type and mutant TrpRS minimal catalytic domains, structures, overview | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Geobacillus stearothermophilus | - |
AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-tagged wild-type and mutant enzymes and isolated catalytic domains from Escherichia coli strain BL21(DE3) soluble fraction by anti-FALG immunoaffinity chromatography and dialysis | Geobacillus stearothermophilus |
Renatured (Comment) | Organism |
---|---|
recombinant FLAG-tagged wild-type and mutant enzymes and isolated catalytic domains from Escherichia coli strain BL21(DE3) inclusion bodies by dilution in solubilization buffer containing 20 mM HEPES, pH 7.8, 6 M urea, 50 mM or 250 mM KCl, 5 mM MgCl2, 1 mM EDTA, 1 mM PMSF, 1 mM 2-mercaptoethanol, 5% glycerol, and dialysis | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? | |
additional information | TrpRS MCD catalytic activity verifies a key prediction of the Rodin-Ohno hypothesis, overview | Geobacillus stearothermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | primary sequence analysis and comparison to other class I and to class II aminoacyl-tRNA transferases, aaRS domain organization and sequence conservation and the sense/antisense coding hypothesis, overview | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the class I aminoacyl-tRNA transferases | Geobacillus stearothermophilus |
TrpRS | - |
Geobacillus stearothermophilus |
Tryptophanyl-tRNA synthetase | - |
Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Geobacillus stearothermophilus |