Cloned (Comment) | Organism |
---|---|
isozyme hmtTrpRS, DNA and amino acid sequence determination and analysis, expression as C-terminally His-tagged enzyme in Spodoptera frugiperda Sf9 and HighFive BTI-NT-5B1-4 insect cells via baculovirus infection, expression as GFP-fusion protein in human 293 cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
L-tryptophan | pH 9.0, 22°C, recombinant enzyme, exchange assay | Homo sapiens | |
0.4 | - |
ATP | pH 9.0, 22°C, recombinant enzyme, exchange assay | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, recombinant His-tagged enzyme, expressed in insect cells, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Homo sapiens | - |
AMP + diphosphate + L-tryptophan-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UGM6 | mitochondrial specific isozyme containing a mitochondrial localization signal sequence | - |
Purification (Comment) | Organism |
---|---|
recombinant from insect cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Homo sapiens | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
L-tryptophan + ATP | the enzyme also catalyzes the exchange of diphosphate in the diphosphate-ATP exchange assay | Homo sapiens | L-Trp-adenylate + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, recombinant His-tagged enzyme, expressed in insect cells, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TrpRS | - |
Homo sapiens |
Tryptophanyl-tRNA synthase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
exchange assay at, room temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
L-tryptophan | pH 9.0, 22°C, recombinant enzyme, exchange assay | Homo sapiens | |
1.4 | - |
ATP | pH 9.0, 22°C, recombinant enzyme, exchange assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
exchange assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |