Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 50 mM HEPES (pH 7.2), 100 mM sodium acetate, 22% (w/v) PEG 3350 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D118A | the mutation results in a 2fold increase in binding affinity | Escherichia coli |
D317A | inactive | Escherichia coli |
R324A | inactive | Escherichia coli |
Y313A | the mutation results in a merely 2fold reduction in binding affinity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | Escherichia coli | - |
AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P11875 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA affinity resin column chromatography and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Escherichia coli | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Arginyl-tRNA synthetase | - |
Escherichia coli |
ArgRS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |