Cloned (Comment) | Organism |
---|---|
quantitative real-time PCR enzyme expression analysis, recombinant expression of tagged wild-type and mutant enzymes in Escherichia coli strain Escherichia coli BL21-CodonPlus (DE3)-RIPL | Homo sapiens |
quantitative real-time PCR enzyme expression analysis, recombinant expression of tagged wild-type and mutant enzymes in Escherichia coli strain Escherichia coli BL21-CodonPlus (DE3)-RIPL | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | siRNA-mediated enzyme knockout in HEK-293T cells. Cells in which EPRS is knocked down show considerable attenuation of the production of antiviral cytokines (IFN-beta and interleukin-6) following viral infection or treatment with the synthetic double-stranded RNA poly(I:C). Activation of the interferon-related signaling molecules IRF3 and STAT1 is significantly lower in cells in which EPRS is knocked down than in their EPRS-sufficient counterparts | Homo sapiens |
additional information | siRNA-mediated enzyme knockout in RAW-264.7 cells. Activation of the interferon-related signaling molecules IRF3 and STAT1 is significantly lower in cells in which EPRS is knocked down than in their EPRS-sufficient counterparts . RAW-264.7 cells stably overexpressing EPRS show significantly less viral replication and more production of IFN-beta and interleukin-6 following infection with PR8 or VSV than those of their counterparts with basal expression of EPRS | Mus musculus |
S990A | site-directed mutagenesis, the mutant is unable to rescue virus-infected cells | Homo sapiens |
S990A | site-directed mutagenesis, the mutant is unable to rescue virus-infected cells | Mus musculus |
S990D | site-directed mutagenesis, the mutation markedly inhibits viral replication in cells | Homo sapiens |
S990D | site-directed mutagenesis, the mutation markedly inhibits viral replication in cells | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
cytoplasm | - |
Mus musculus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlu | Homo sapiens | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | Mus musculus | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | Mus musculus C57BL/6 | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07814 | - |
- |
Mus musculus | Q8CGC7 | - |
- |
Mus musculus C57BL/6 | Q8CGC7 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | infection-specific phosphorylation of EPRS at a Ser induces its dissociation from the MSC, after which it is guided to the antiviral signaling pathway, where it interacts with PCBP2, a negative regulator of mitochondrial antiviral signaling protein (MAVS) that is critical for antiviral immunity | Homo sapiens |
phosphoprotein | infection-specific phosphorylation of EPRS at Ser990 induces its dissociation from the MSC, after which it is guided to the antiviral signaling pathway, where it interacts with PCBP2, a negative regulator of mitochondrial antiviral signaling protein (MAVS) that is critical for antiviral immunity | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant tagged wild-type and mutant enzymes from Escherichia coli strain Escherichia coli BL21-CodonPlus (DE3)-RIPL by affinity chromatography and gel filtration | Homo sapiens |
recombinant tagged wild-type and mutant enzymes from Escherichia coli strain Escherichia coli BL21-CodonPlus (DE3)-RIPL by affinity chromatography and gel filtration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
macrophage | - |
Homo sapiens | - |
macrophage | - |
Mus musculus | - |
RAW-264.7 cell | - |
Mus musculus | - |
U-937 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlu | - |
Homo sapiens | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | - |
Mus musculus | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | - |
Mus musculus C57BL/6 | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is part of a multi-tRNA synthetase complex (MSC) | Homo sapiens |
More | the enzyme is part of a multi-tRNA synthetase complex (MSC) | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
bifunctional aminoacyl-tRNA synthetase | UniProt | Homo sapiens |
bifunctional aminoacyl-tRNA synthetase | UniProt | Mus musculus |
bifunctional glutamate/proline-tRNA ligase | UniProt | Homo sapiens |
bifunctional glutamate/proline-tRNA ligase | UniProt | Mus musculus |
EPRS | - |
Homo sapiens |
EPRS | - |
Mus musculus |
glutamyl-prolyl tRNA synthetase | - |
Homo sapiens |
glutamyl-prolyl tRNA synthetase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Mus musculus |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | requirement for sensing by the immune system in the induction of EPRS expression during infection with an RNA virus | up |
Mus musculus | requirement for sensing by the immune system in the induction of EPRS expression during infection with an RNA virus, VSV Indiana strain | up |
General Information | Comment | Organism |
---|---|---|
malfunction | EPRS-haploid (Eprs+/-) mice show enhanced viremia and inflammation and delayed viral clearance | Mus musculus |
metabolism | under conditions of stress, several MSC components, including EPRS, methionyl-tRNA synthetase (MRS), lysyl-tRNA synthetase (KRS), AIMP1 and AIMP2, are released from the complex through post-translational modifications to exert activities during non-translational events such as inflammation, cell metabolism, angiogenesis, and tumorigenesis. Phosphorylation is the critical regulatory mechanism that determines the non-translational function of ARSs in cells, overview | Homo sapiens |
metabolism | under conditions of stress, several MSC components, including EPRS, methionyl-tRNA synthetase (MRS), lysyl-tRNA synthetase (KRS), AIMP1 and AIMP2, are released from the complex through post-translational modifications to exert activities during non-translational events such as inflammation, cell metabolism, angiogenesis, and tumorigenesis. Phosphorylation is the critical regulatory mechanism that determines the non-translational function of ARSs in cells, overview | Mus musculus |
additional information | the enzyme is part of a multi-tRNA synthetase complex (MSC) | Homo sapiens |
additional information | the enzyme is part of a multi-tRNA synthetase complex (MSC) | Mus musculus |
physiological function | the multi-tRNA synthetase complex (MSC) component glutamyl-prolyl-tRNA synthetase (EPRS) switched its function following viral infection and exhibited potent antiviral activity. Infection-specific phosphorylation of EPRS at a Ser induces its dissociation from the MSC, after which it is guided to the antiviral signaling pathway, where it interacts with PCBP2, a negative regulator of mitochondrial antiviral signaling protein (MAVS) that is critical for antiviral immunity. EPRS protects MAVS from PCBP2-mediated ubiquitination. The stimulus-inducible activation of MAVS by enzyme EPRS suggests an unexpected role for the MSC as a regulator of immune responses to viral infection. Phosphorylation of EPRS at a Ser is the driving force that leads to the antiviral roles of EPRS in regulating MAVS | Homo sapiens |
physiological function | the multi-tRNA synthetase complex (MSC) component glutamyl-prolyl-tRNA synthetase (EPRS) switched its function following viral infection and exhibited potent antiviral activity. Infection-specific phosphorylation of EPRS at Ser990 induces its dissociation from the MSC, after which it is guided to the antiviral signaling pathway, where it interacts with PCBP2, a negative regulator of mitochondrial antiviral signaling protein (MAVS) that is critical for antiviral immunity. EPRS protects MAVS from PCBP2-mediated ubiquitination. The stimulus-inducible activation of MAVS by enzyme EPRS suggests an unexpected role for the MSC as a regulator of immune responses to viral infection. Phosphorylation of EPRS at Ser990 is the driving force that leads to the antiviral roles of EPRS in regulating MAVS | Mus musculus |