Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with 5'-O-(N-(L-seryl)-sulfamoyl)adenosine, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M ammonium fluoride or ammonium formate, and 0.1 M HEPES pH 7.0 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTAG254-N261 | the mutant shows 72% activity compared to the wild type enzyme | Homo sapiens |
DELTAG75-N97 | the mutant shows 13% activity compared to the wild type enzyme | Homo sapiens |
DELTAG75-N97/DELTAG254-N261 | the mutant shows 7% activity compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
tRNASer | mutant enzyme DELTAG75-N97/DELTAG254-N261, at pH 7.5 and 37°C | Homo sapiens | |
0.00033 | - |
tRNASer | mutant enzyme DELTAG75-N97, at pH 7.5 and 37°C | Homo sapiens | |
0.00051 | - |
tRNASer | mutant enzyme DELTAG254-N261, at pH 7.5 and 37°C | Homo sapiens | |
0.00077 | - |
tRNASer | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Homo sapiens | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49591 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Homo sapiens | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SerRS | - |
Homo sapiens |
Seryl-tRNA synthetase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
tRNASer | mutant enzyme DELTAG75-N97/DELTAG254-N261, at pH 7.5 and 37°C | Homo sapiens | |
0.025 | - |
tRNASer | mutant enzyme DELTAG75-N97, at pH 7.5 and 37°C | Homo sapiens | |
0.2 | - |
tRNASer | mutant enzyme DELTAG254-N261, at pH 7.5 and 37°C | Homo sapiens | |
0.43 | - |
tRNASer | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | seryl-tRNA synthetase is an essential enzyme for translation, also regulating vascular development | Homo sapiens |