Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Trypanosoma brucei |
Crystallization (Comment) | Organism |
---|---|
in complex with L-methionine and inhibitors, sitting drop vapor diffusion method, using 2.0-2.3 M (NH4)2SO4, 0.2 M NaCl, and 0.1 M sodium cacodylate (pH 6.0-6.6) | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-([3-[(3,5-dibromo-2-ethoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one | - |
Trypanosoma brucei | |
2-([3-[(3,5-dibromo-2-methoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one | - |
Trypanosoma brucei | |
2-([3-[(3,5-dichlorobenzyl)amino]propyl]amino)-4a,8a-dihydroquinolin-4(1H)-one | - |
Trypanosoma brucei | |
2-aminoquinolin-8-ol | - |
Trypanosoma brucei | |
4-[4-[(1H-benzimidazol-2-ylmethyl)amino]-6-(2-chloro-4-methoxyphenoxy)pyrimidin-2-yl]piperazin-2-one | - |
Trypanosoma brucei | |
N-(1H-benzimidazol-2-yl)-N'-(3,5-dichlorobenzyl)propane-1,3-diamine | - |
Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | Trypanosoma brucei | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | Q38C91 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography and Superdex 75 gel filtration | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Trypanosoma brucei | AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionyl-tRNA synthetase | - |
Trypanosoma brucei |
MetRS | - |
Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000008 | - |
pH and temperature not specified in the publication | Trypanosoma brucei | 2-([3-[(3,5-dichlorobenzyl)amino]propyl]amino)-4a,8a-dihydroquinolin-4(1H)-one | |
0.000013 | - |
pH and temperature not specified in the publication | Trypanosoma brucei | 2-([3-[(3,5-dibromo-2-ethoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one | |
0.000043 | - |
pH and temperature not specified in the publication | Trypanosoma brucei | 4-[4-[(1H-benzimidazol-2-ylmethyl)amino]-6-(2-chloro-4-methoxyphenoxy)pyrimidin-2-yl]piperazin-2-one | |
0.000046 | - |
pH and temperature not specified in the publication | Trypanosoma brucei | N-(1H-benzimidazol-2-yl)-N'-(3,5-dichlorobenzyl)propane-1,3-diamine | |
0.000065 | - |
pH and temperature not specified in the publication | Trypanosoma brucei | 2-([3-[(3,5-dibromo-2-methoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one |