Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.1 extracted from

  • Druart, K.; Guennec, M.L.; Palmai, Z.; Simonson, T.
    Probing the stereospecificity of tyrosyl- and glutaminyl-tRNA synthetase with molecular dynamics (2017), J. Mol. Graph. Model., 71, 192-199 .
    View publication on PubMed
Search for more literature for 6.1.1.1

Protein Variants

Protein Variants Comment Organism
D81R site-diretced mutagenesis, the mutant shows reduced stereospecificity for L-Tyr compared to wild-type Methanocaldococcus jannaschii
E36Q site-diretced mutagenesis, the mutant shows increased, but not inverted, stereospecificity for L-Tyr compared to wild-type Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tyrosine + tRNATyr Methanocaldococcus jannaschii
-
 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
ATP + L-tyrosine + tRNATyr Methanocaldococcus jannaschii TCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440
-
 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57834 i.e. Methanocaldococcus jannaschii
-
Methanocaldococcus jannaschii TCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440 Q57834 i.e. Methanocaldococcus jannaschii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
ATP + L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii TCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
additional information enzyme TyrRS has a detectable, natural, tRNA-acylation activity for the D-tyrosine stereoisomer, being capable of charging D-Tyr onto tRNATyr to form D-Tyr-tRNA instead of the usual L-Tyr-tRNA Methanocaldococcus jannaschii ?
-
 ?
additional information enzyme TyrRS has a detectable, natural, tRNA-acylation activity for the D-tyrosine stereoisomer, being capable of charging D-Tyr onto tRNATyr to form D-Tyr-tRNA instead of the usual L-Tyr-tRNA Methanocaldococcus jannaschii TCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440 ?
-
 ?

Synonyms

Synonyms Comment Organism
class I tyrosyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
Tyrosyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
TyrRS
-
 Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanocaldococcus jannaschii

General Information

General Information Comment Organism
evolution the enzyme belongs to the class I aminoacyl-tRNA synthetase family Methanocaldococcus jannaschii
additional information molecular dynamics modeling of substrates L- and D-Tyr into the active site of wild-type enzyme and mutants D81R and E36Q using the PDB ID 1J1U X-ray structure, superimposed based on their protein/tRNA environment, enzyme molecular dynamics simulation amd modeling, structure-function analysis, detailed overview Methanocaldococcus jannaschii