Literature summary for 5.6.1.8 extracted from

Katayama, T.; Watanabe, M.; Tanaka, H.; Hino, M.; Miyakawa, T.; Ohki, T.; Ye, L.; Xie, C.; Yoshiyama, S.; Nakamura, A.; Ishikawa, R.; Tanokura, M.; Oiwa, K.; Kohama, K.
Stimulatory effects of arachidonic acid on myosin ATPase activity and contraction of smooth muscle via myosin motor domain (2010), Am. J. Physiol. Heart Circ. Physiol., 298, H505-H514.

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Activating Compound

Activating Compound	Comment	Organism	Structure
arachidonic acid	0.0503 mM half-maximal effective concentration, 0.0263 mM half-maximal effective concentration in the presence of actin	Gallus gallus

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged myosin head (Met1-Gln729) expressed in a baculovirus system	Gallus gallus

Organism

Organism	UniProt	Comment	Textmining
Gallus gallus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
myosin with unphosphorylated regulatory light chain (20000 Da) from smooth muscle of gizzard; recombinant His-tagged myosin head: immobilized metal ion affinity chromatography (Ni2+)	Gallus gallus

Source Tissue

Source Tissue	Comment	Organism	Textmining
gizzard smooth muscle	-	Gallus gallus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + myosin bound to actin filament at position n	-	Gallus gallus	ADP + phosphate + myosin bound to actin filament at position n+1	-	?
CTP + H2O + myosin bound to actin filament at position n	-	Gallus gallus	CDP + phosphate + myosin bound to actin filament at position n+1	-	?

Synonyms

Synonyms	Comment	Organism
myosin ATPase	-	Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00892	-	ATP	unphosphorylated myosin, pH 7.5, 25°C	Gallus gallus
0.0462	-	ATP	actin-activated, unphosphorylated myosin, pH 7.5, 25°C	Gallus gallus
0.0678	-	ATP	phosphorylated myosin, pH 7.5, 25°C	Gallus gallus
0.259	-	ATP	actin-activated, phosphorylated myosin, pH 7.5, 25°C	Gallus gallus

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Release 2023.1 (January 2023)