Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Gold cells | Chlamydomonas reinhardtii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 8% (w/v) PEG 6000, 0.15 M NaCl, and 0.1 M Tris-HCl pH 8.0, at 18°C | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Chlamydomonas reinhardtii | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | Chlamydomonas reinhardtii | - |
ADP + phosphate + an unfolded polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | A8JE91 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | - |
Chlamydomonas reinhardtii | ADP + phosphate + an unfolded polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooligomer | x * 60000, SDS-PAGE | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
chaperonin 60B1 | - |
Chlamydomonas reinhardtii |
CPN60beta1 | subunit | Chlamydomonas reinhardtii |