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Literature summary for 5.6.1.7 extracted from

  • Nakamura, N.; Taguchi, H.; Ishii, N.; Yoshida, M.; Suzuki, M.; Endo, I.; Miura, K.; Yohda, M.
    Purification and molecular cloning of the group II chaperonin from the acidothermophilic archaeon, Sulfolobus sp. strain 7 (1997), Biochem. Biophys. Res. Commun., 236, 727-732.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
visualization of enzyme by transmission electron microscopy shows a ring complex, with a diameter of the ring of 16.7 nm, and a dark cavity of 5 nm diameter present when viewed from the top Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
x * 56000, alpha subunit, + x * 56000, beta subunit, SDS-PAGE Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii O24734 alpha subunit of thermosome
-
Sulfurisphaera tokodaii O24735 beta subunit of thermosome
-
Sulfurisphaera tokodaii 7 O24734 alpha subunit of thermosome
-
Sulfurisphaera tokodaii 7 O24735 beta subunit of thermosome
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
70°C Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a folded polypeptide
-
Sulfurisphaera tokodaii ADP + phosphate + an unfolded polypeptide
-
?
ATP + H2O + a folded polypeptide
-
Sulfurisphaera tokodaii 7 ADP + phosphate + an unfolded polypeptide
-
?
additional information enzyme inhibits the spontaneous refolding of lactate dehydrogenase denatured with 6.4 M guanidine HCl. The addition of ATP does not enhance the degree of refolding Sulfurisphaera tokodaii ?
-
?
additional information enzyme inhibits the spontaneous refolding of lactate dehydrogenase denatured with 6.4 M guanidine HCl. The addition of ATP does not enhance the degree of refolding Sulfurisphaera tokodaii 7 ?
-
?

Subunits

Subunits Comment Organism
? x * 56000, alpha subunit, + x * 56000, beta subunit, SDS-PAGE Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
thermosome
-
Sulfurisphaera tokodaii