Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Methanocaldococcus jannaschii | |
N-ethylmaleimide | - |
Methanocaldococcus jannaschii | |
p-chloromercuriphenyl-sulfonic acid | - |
Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
ATP | HslU | Escherichia coli | |
0.307 | - |
CTP | PAN | Methanocaldococcus jannaschii | |
0.477 | - |
ATP | PAN(DELTA1-73) | Methanocaldococcus jannaschii | |
0.493 | - |
CTP | PAN(DELTA1-73) | Methanocaldococcus jannaschii | |
0.497 | - |
ATP | PAN | Methanocaldococcus jannaschii | |
0.55 | - |
ATP | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
650000 | - |
PAN, proteasome-activating nucleotidase, complex with ATPase activity, gel filtration | Methanocaldococcus jannaschii |
700000 | - |
- |
Saccharomyces cerevisiae |
700000 | - |
- |
Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + polypeptide | Escherichia coli | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | Saccharomyces cerevisiae | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | Trypanosoma brucei | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | Rhodococcus erythropolis | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | Trichormus variabilis | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | Methanocaldococcus jannaschii | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | Methanosarcina thermophila | - |
ADP + phosphate + unfolded polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Methanocaldococcus jannaschii | - |
- |
- |
Methanosarcina thermophila | - |
- |
- |
Rhodococcus erythropolis | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Trichormus variabilis | - |
- |
- |
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
700-kDa complex | Methanocaldococcus jannaschii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Escherichia coli | |
ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Saccharomyces cerevisiae | |
ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Trypanosoma brucei | |
ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Rhodococcus erythropolis | |
ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Trichormus variabilis | |
ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Methanocaldococcus jannaschii | |
ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + polypeptide | - |
Escherichia coli | ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | - |
Saccharomyces cerevisiae | ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | - |
Trypanosoma brucei | ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | - |
Rhodococcus erythropolis | ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | - |
Trichormus variabilis | ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | ADP + phosphate + unfolded polypeptide | - |
? | |
ATP + H2O + polypeptide | - |
Methanosarcina thermophila | ADP + phosphate + unfolded polypeptide | - |
? | |
CTP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | CDP + phosphate + unfolded polypeptide | - |
? | |
GTP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | GDP + phosphate + unfolded polypeptide | - |
? | |
ITP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | IDP + phosphate + unfolded polypeptide | - |
? | |
TTP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | TDP + phosphate + unfolded polypeptide | - |
? | |
UTP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | UDP + phosphate + unfolded polypeptide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
PAN(DELTA1-73) | Methanocaldococcus jannaschii |
80 | - |
full-length protein | Methanocaldococcus jannaschii |