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Literature summary for 5.6.1.3 extracted from

  • Song, H.; Endow, S.A.
    Binding sites on microtubules of kinesin motors of the same or opposite polarity (1996), Biochemistry, 35, 11203-11209.
    View publication on PubMed
Search for more literature for 5.6.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Drosophila sp. (in: flies)
KHC cloned and expressed in Escherichia coli BL21(DE3)pLysS Drosophila sp. (in: flies)

Localization

Localization Comment Organism GeneOntology No. Textmining
microtubule binding site of plus-end-directed kinesin heavy chain (KHC) on microtubule Drosophila sp. (in: flies) 5874
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37360
-
 1 * 37360, KHC, molecular mass calculated from amino acid sequence predicted from nucleic acid sequence Drosophila sp. (in: flies)
90000
-
 gel filtration Drosophila sp. (in: flies)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n Drosophila sp. (in: flies)
-
 ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
ATP + H2O + a kinesin associated with a microtubule at position n Xenopus laevis
-
 ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
GTP + H2O + a kinesin associated with a microtubule at position n Drosophila sp. (in: flies)
-
 GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
GTP + H2O + a kinesin associated with a microtubule at position n Xenopus laevis
-
 GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila sp. (in: flies)
-
-
-
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, expressed in Escherichia coli Drosophila sp. (in: flies)

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) microtubular motor protein, involved in organelle movement, in mitosis and meiosis. In contrast to dynein, it moves along microtubules towards the plus end. Composed of two heavy (a) chains (110kDa) and two more light (b) chains (65-75 kDa). Also hydrolyzes GTP. Drosophila sp. (in: flies)
a
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) microtubular motor protein, involved in organelle movement, in mitosis and meiosis. In contrast to dynein, it moves along microtubules towards the plus end. Composed of two heavy (a) chains (110kDa) and two more light (b) chains (65-75 kDa). Also hydrolyzes GTP. Xenopus laevis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila sp. (in: flies) ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
ATP + H2O + a kinesin associated with a microtubule at position n
-
 Xenopus laevis ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
GTP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila sp. (in: flies) GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
GTP + H2O + a kinesin associated with a microtubule at position n
-
 Xenopus laevis GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 37360, KHC, molecular mass calculated from amino acid sequence predicted from nucleic acid sequence Drosophila sp. (in: flies)

Synonyms

Synonyms Comment Organism
Eg5
-
 Xenopus laevis
KHC
-
 Drosophila sp. (in: flies)