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Literature summary for 5.6.1.1 extracted from
- Spastin oligomerizes into a hexamer and the mutant spastin (E442Q) redistribute the wild-type spastin into filamentous microtubule (2008), J. Neurochem., 106, 613-624.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | spastin is frequently mutated in hereditary spastic paraplegia | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the human short isoform of spastin initiating from the alternative start codon is amplified by PCR and cloned into the pET41a vector for expression in Escherichia coli BL21DE3 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E442Q | missense mutation | Homo sapiens |
| R499C | missense mutation | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.497 | - |
ATP | - |
Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | short isoform, lacks putative transmembrane motif | Homo sapiens | 5737 | - |
| nucleus | short isoform, lacks putative transmembrane motif | Homo sapiens | 5634 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
monomer, GST-spastin, determined by gel filtration analysis and Western blotting | Homo sapiens |
| 350000 | - |
hexamer, GST-free spastin, determined by gel filtration analysis and Western blotting | Homo sapiens |
| 530000 | - |
hexamer, GST-spastin, determined by gel filtration analysis and Western blotting | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a glutathione-agarose column, the glutathione-S-transferase moiety is cleaved with thrombin | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| spastin | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
ATPase activity assay | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
ATPase activity assay | Homo sapiens |
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Release 2023.1 (January 2023)
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