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Literature summary for 5.5.1.7 extracted from
- Structural basis for the substrate specificity and the absence of dehalogenation activity in 2-chloromuconate cycloisomerase from Rhodococcus opacus 1CP (2014), Biochim. Biophys. Acta, 1844, 1541-1549.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the crystallographic structure of the homooctameric Rho-2-CMCI is solved at 2.5 A resolution by molecular replacement using the coordinates of the structure of chloromuconate cycloisomerase from Pseudomonas putida PRS2000 | Rhodococcus opacus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0487 | - |
3-methyl-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus |  |
| 0.0559 | - |
2-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
| 0.0813 | - |
cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
| 1.24 | - |
3-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus opacus | Q8G9L1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anion-exchange chromatography on DEAE-Toyopearl, hydrophobic chromatography on Phenyl-sepharose with linear gradient (NH4)2SO4, anion-exchange Q3 chromatography and hydrophobic chromatography | Rhodococcus opacus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Chloro-cis,cis-muconate | - |
Rhodococcus opacus | 4-Carboxymethyl-2-chloro-but-2-en-4-olide | - |
? | |
| 3-Chloro-cis,cis-muconate | - |
Rhodococcus opacus | cis-4-Carboxymethylenebut-2-en-4-olide | - |
? | |
| 3-Methyl-cis,cis-muconate | - |
Rhodococcus opacus | ? | - |
? | |
| cis,cis-Muconate | - |
Rhodococcus opacus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homooctamer | - |
Rhodococcus opacus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-chloromuconate cycloisomerase | - |
Rhodococcus opacus |
| Rho-2-CMCl | - |
Rhodococcus opacus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
3-methyl-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
| 3.6 | - |
2-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
| 8.8 | - |
3-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
| 24.66 | - |
cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.66 | - |
3-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
| 65 | - |
2-chloro-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
| 65 | - |
3-methyl-cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
| 303 | - |
cis,cis-muconate | pH and temperature not specified in the publication | Rhodococcus opacus | |
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