Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Nepeta racemosa |
Protein Variants | Comment | Organism |
---|---|---|
K171M | isoform Neps1, loss of activity | Nepeta racemosa |
L156S | isoform Neps1, loss of activity | Nepeta racemosa |
N125A | isoform Neps1, partial loss of activity | Nepeta racemosa |
P155S | isoform Neps1, partial loss of activity | Nepeta racemosa |
S198M | isoform Neps1, loss of activity | Nepeta racemosa |
T152N | isoform Neps1, loss of activity | Nepeta racemosa |
T153A | isoform Neps1, activity similar to wild-type | Nepeta racemosa |
T154G | isoform Neps1, improved activity | Nepeta racemosa |
T202A | isoform Neps1, loss of activity | Nepeta racemosa |
V199A | isoform Neps1, activity similar to wild-type | Nepeta racemosa |
Y167F | isoform Neps1, loss of activity | Nepeta racemosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | 0.1 M NaCl used in assay conditions | Nepeta racemosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-8-oxocitronellyl enol | Nepeta racemosa | - |
(+)-cis,trans-nepetalactol | - |
? | |
8-oxocitronellyl enol | Nepeta racemosa | - |
(+)-cis,trans-nepetalactol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nepeta racemosa | - |
- |
- |
Nepeta racemosa | A0A3Q8GL18 | catmint | - |
Nepeta racemosa | A0A3Q8GYY4 | catmint | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, HisTrap column chromatography and Superdex 200 gel filtration | Nepeta racemosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nepeta racemosa | - |
trichome | glandular trichome | Nepeta racemosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-8-oxocitronellyl enol | - |
Nepeta racemosa | (+)-cis,trans-nepetalactol | - |
? | |
8-oxocitronellyl enol | - |
Nepeta racemosa | (+)-cis,trans-nepetalactol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nepetalactol-related SDR 1 | - |
Nepeta racemosa |
nepetalactol-related short-chain dehydrogenase/reductase 1 | - |
Nepeta racemosa |
nepetalactol-related short-chain dehydrogenase/reductase 2 | - |
Nepeta racemosa |
NEPS1 | - |
Nepeta racemosa |
NEPS2 | - |
Nepeta racemosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Nepeta racemosa |
General Information | Comment | Organism |
---|---|---|
physiological function | isoforms Neps1 and Neps2 catalyze the stereoselective cyclisation of reactive intermediate (S)-8-oxocitronellyl enol into nepetalactol. Subsequent oxidation of nepetalactol by NEPS1 provides nepetalactone, metabolites known for both insect-repellent activity and euphoric effect in cats | Nepeta racemosa |