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Literature summary for 5.4.99.8 extracted from

  • Gas-Pascual, E.; Simonovik, B.; Heintz, D.; Bergdoll, M.; Schaller, H.; Bach, T.J.
    Inhibition of cycloartenol synthase (CAS) function in tobacco BY-2 cell suspensions a proteomic analysis (2015), Lipids, 50, 773-784 .
    View publication on PubMed
Search for more literature for 5.4.99.8

Cloned(Commentary)

Cloned (Comment) Organism
gene CAS, recombinant expression in TBY-2 cells, usage of Agrobacterium tumefaciens strain LBA4404 hyper-virulent strain and transformation into BY-2 cell suspensions via co-culture, quantitative reverse transcriptase-mediated real-time PCR enzyme expression analysis. After 6 days of treatment, the expression of NtCAS1 increases 2.5fold in the BY-2 cells Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
Ro 48-8071 a potent inhibitor of oxidosqualene cyclization, the site of RO 48-8071 inhibition is CAS Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene Nicotiana tabacum
-
 cycloartenol
-
 r

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum A0A0A1ER86 cv. BY-2
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Nicotiana tabacum
-
leaf
-
 Nicotiana tabacum
-
TBY-2 cell
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
 Nicotiana tabacum cycloartenol
-
 r

Synonyms

Synonyms Comment Organism
NtCAS
-
 Nicotiana tabacum

Expression

Organism Comment Expression
Nicotiana tabacum inhibitor RO 48-8071 leads to downregulation of gene CAS in cells. CAS gene expression is partially blocked by using an inducible artificial microRNA with limited effectiveness down

General Information

General Information Comment Organism
malfunction in vivo application of inhibitor RO 48-8071 TBY-2 cells in short-term treatments (24 h) results in accumulation of oxidosqualene with no changes in the final sterol products. Long-term treatments (6 days) induces downregulation in gene expression not only of CAS but also of the SMT2 gene coding sterol methyltransferase 2 (EC 2.1.1.41) explaining some of the increase in 24-methyl sterols at the expense of the 24-ethyl sterols stigmasterol and sitosterol Nicotiana tabacum
metabolism building blocks for plant sterol biosynthesis, involving enzyme CAS, are provided by the mevalonate (MVA) pathway, overview Nicotiana tabacum