Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli KA13 cells | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
D48G | the mutation causes kcat/Km to decrease by 3 fold | Methanocaldococcus jannaschii |
F77W | mutant exhibits decreased activity compared to the wild type enzyme | Methanocaldococcus jannaschii |
Q88N | mutant exhibits decreased activity compared to the wild type enzyme | Methanocaldococcus jannaschii |
R51Q | mutant exhibits decreased activity compared to the wild type enzyme | Methanocaldococcus jannaschii |
V35A | mutant exhibits decreased activity compared to the wild type enzyme | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
chorismate | mutant enzyme V35A, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii | |
0.054 | - |
chorismate | wild type enzyme, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii | |
0.059 | - |
chorismate | mutant enzyme D48G, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii | |
0.073 | - |
chorismate | mutant enzyme F77W, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii | |
1.2 | - |
chorismate | mutant enzyme Q88N, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii | |
1.3 | - |
chorismate | mutant enzyme R51Q, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography and gel filtration | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | - |
Methanocaldococcus jannaschii | Prephenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
chorismate | mutant enzyme Q88N, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii | |
0.41 | - |
chorismate | mutant enzyme F77W, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii | |
0.5 | - |
chorismate | mutant enzyme V35A, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii | |
1.3 | - |
chorismate | mutant enzyme D48G, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii | |
2.6 | - |
chorismate | mutant enzyme R51Q, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii | |
3 | - |
chorismate | wild type enzyme, in PBS buffer (pH 7.5) at 20°C | Methanocaldococcus jannaschii |