Literature summary for 5.4.99.47 extracted from

Segura, M.J.; Lodeiro, S.; Meyer, M.M.; Patel, A.J.; Matsuda, S.P.
Directed evolution experiments reveal mutations at cycloartenol synthase residue His477 that dramatically alter catalysis (2002), Org. Lett., 4, 4459-4462.

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Protein Variants

Protein Variants	Comment	Organism
H477N	the mutant produces 88% lanosterol and 12% parkeol from oxidosqualene	Arabidopsis thaliana
H477Q	the mutant produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol from oxidosqualene	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
oxidosqualene	-	Arabidopsis thaliana	lanosterol + parkeol	mutant H477N produces 88% lanosterol and 12% parkeol	?
oxidosqualene	-	Arabidopsis thaliana	lanosterol + parkeol	mutant H477Q produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol	?

Synonyms

Synonyms	Comment	Organism
cycloartenol synthase mutant H477N	cf. EC 5.4.99.8	Arabidopsis thaliana
cycloartenol synthase mutant H477Q	cf. EC 5.4.99.8	Arabidopsis thaliana

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Release 2023.1 (January 2023)