BRENDA - Enzyme Database
show all sequences of 5.4.99.41

Molecular cloning and expression in yeast of 2,3-oxidosqualene-triterpenoid cyclases from Arabidopsis thaliana

Husselstein-Muller, T.; Schaller, H.; Benveniste, P.; Plant Mol. Biol. 45, 75-92 (2001)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Saccharomyces cerevisiae
Arabidopsis thaliana
Engineering
Protein Variants
Commentary
Organism
additional information
functional expression in Saccharomyces cerevisiae lacking lanosterol synthase activity results in production of lupeol
Arabidopsis thaliana
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q9C5M3
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(3S)-oxidosqualene
-
683983
Arabidopsis thaliana
lupeol
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
2,3-oxidosqualene-triterpenoid cyclase
-
Arabidopsis thaliana
AtLUP1
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Arabidopsis thaliana
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
functional expression in Saccharomyces cerevisiae lacking lanosterol synthase activity results in production of lupeol
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(3S)-oxidosqualene
-
683983
Arabidopsis thaliana
lupeol
-
-
-
?
Other publictions for EC 5.4.99.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749117
Loeschcke
The photosynthetic bacteria R ...
Arabidopsis thaliana
PLoS ONE
12
e0189816
2017
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748772
Andre
Multifunctional oxidosqualene ...
Malus domestica
New Phytol.
211
1279-1294
2016
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730247
Pena-Rodriguez
Isotopologue profiling of trit ...
Pentalinon andrieuxii
J. Org. Chem.
79
2864-2873
2014
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748877
Nasrollahi
The effect of drought stress ...
Glycyrrhiza glabra
Phytochemistry
103
32-37
2014
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730620
Fan
-
Gene expression of lupeol synt ...
Betula platyphylla
Plant Mol. Biol. Rep.
31
296-302
2013
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683462
Basyuni
Triterpene synthases from the ...
Bruguiera gymnorhiza
FEBS J.
274
5028-5042
2007
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677806
Guhling
Cloning and characterization o ...
Ricinus communis
Arch. Biochem. Biophys.
448
60-72
2006
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1
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682450
Sawai
-
Functional and structural anal ...
Lotus japonicus
Plant Sci.
170
247-257
2006
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683951
Kushiro
Stereochemical course in water ...
Arabidopsis thaliana
Org. Lett.
8
5589-5592
2006
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683585
Shan
Enzymatic cyclization of dioxi ...
Arabidopsis thaliana
J. Am. Chem. Soc.
127
18008-18009
2005
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683803
Xiong
Structure and reactivity of th ...
Arabidopsis thaliana
J. Org. Chem.
70
5362-5375
2005
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683215
Zhang
Oxidosqualene cyclases from ce ...
Betula platyphylla
Biol. Pharm. Bull.
26
642-650
2003
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683582
Xiong
Enzymatic synthesis of an indo ...
Arabidopsis thaliana
J. Am. Chem. Soc.
125
9002-9003
2003
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683983
Husselstein-Muller
Molecular cloning and expressi ...
Arabidopsis thaliana
Plant Mol. Biol.
45
75-92
2001
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683581
Kushiro
-
Mutational studies on triterpe ...
Olea europaea
J. Am. Chem. Soc.
122
6816-6824
2000
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6
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683950
Segura
Arabidopsis thaliana LUP1 conv ...
Arabidopsis thaliana
Org. Lett.
2
2257-2259
2000
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684073
Kushiro
-
A novel multifunctional triter ...
Arabidopsis thaliana
Tetrahedron Lett.
41
7705-7710
2000
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683421
Shibuya
Two branches of the lupeol syn ...
Olea europaea, Taraxacum officinale
Eur. J. Biochem.
266
302-307
1999
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2
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683580
Kushiro
-
Chimeric triterpene synthase. ...
Arabidopsis thaliana
J. Am. Chem. Soc.
121
1208-1216
1999
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684072
Kushiro
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Cryptic regiospecificity in de ...
Arabidopsis thaliana, Olea europaea, Taraxacum officinale
Tetrahedron Lett.
40
5553-5556
1999
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683971
Herrera
Cloning and characterization o ...
Arabidopsis thaliana
Phytochemistry
49
1905-1911
1998
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