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Literature summary for 5.4.99.39 extracted from

  • Um, Y.; Jin, M.; Lee, D.; Kim, C.; Hong, C.; Lee, Y.; Kim, O.
    Functional characterization of the beta-amyrin synthase gene involved in platycoside biosynthesis in Platycodon grandiflorum (2017), Hortic. Environ. Biotechnol., 58, 613-619 .
No PubMed abstract available
Search for more literature for 5.4.99.39

Cloned(Commentary)

Cloned (Comment) Organism
gene bAS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Saccharomyces cerevisiae strain GIL77, real-time RT-PCR enzyme expression analysis, MS analysis metabolite identification Platycodon grandiflorus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene Platycodon grandiflorus
-
 beta-amyrin
-
 r

Organism

Organism UniProt Comment Textmining
Platycodon grandiflorus A0A1Z2WUZ5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Platycodon grandiflorus
-
leaf
-
 Platycodon grandiflorus
-
additional information about equal enzyme expression in the different tissues, quantitative tissue-specific real-time RT-PCR gene expression analysis Platycodon grandiflorus
-
root
-
 Platycodon grandiflorus
-
stem
-
 Platycodon grandiflorus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
 Platycodon grandiflorus beta-amyrin
-
 r
additional information GC-MS analysis metabolite identification Platycodon grandiflorus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 87996, sequence calculation Platycodon grandiflorus

Synonyms

Synonyms Comment Organism
bAS
-
 Platycodon grandiflorus
PlgOSC1
-
 Platycodon grandiflorus

General Information

General Information Comment Organism
evolution PlgOSC1 not only contains a DCTAE motif that is implicated in substrate binding, but also has four repeats of the QXXXGXW motif, which is a typical feature of the triterpene synthase superfamily. PlgOSC1 has a Try residue in the MWCYCR motif that plays a role in the formation of beta-amyrin Platycodon grandiflorus
metabolism the enzyme synthesizes beta-amyrin, a central precursor in the platycoside biosynthesis pathway, overview. 69 platycosides are identified from Platycodon grandiflorum Platycodon grandiflorus