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Literature summary for 5.4.99.39 extracted from

  • Sun, R.; Liu, S.; Tang, Z.Z.; Zheng, T.R.; Wang, T.; Chen, H.; Li, C.L.; Wu, Q.
    beta-Amyrin synthase from Conyza blinii expressed in Saccharomyces cerevisiae (2017), FEBS open bio, 7, 1575-1585 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.39

Cloned(Commentary)

Cloned (Comment) Organism
gene BAS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expressison in Saccharomyces cerevisiae strain INVSc1 under the control of GAL1 promoter yielding beta-amyrin, beta-amyrin biosynthesis pathway engineered in yeast, overview. Metabolite identification by GC-MS/MS analysis Eschenbachia blinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene Eschenbachia blinii
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 beta-amyrin
-
 r

Organism

Organism UniProt Comment Textmining
Eschenbachia blinii A0A2P0VE73 i.e. Conyza blinii, collected in 2014 from Panzhihua, Sichuan, China
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
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 Eschenbachia blinii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
 Eschenbachia blinii beta-amyrin
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 r
additional information metabolite identification by GC-MS/MS analysis Eschenbachia blinii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 87700, about, sequence calculation Eschenbachia blinii

Synonyms

Synonyms Comment Organism
bAS
-
 Eschenbachia blinii
betaAS
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 Eschenbachia blinii
CbbAS
-
 Eschenbachia blinii
OSC
-
 Eschenbachia blinii
Oxidosqualene cyclase
-
 Eschenbachia blinii

Expression

Organism Comment Expression
Eschenbachia blinii the transcription of gene CbbAS is upregulated 4-24 h after treatment of seedlings with methyl jasmonate up

General Information

General Information Comment Organism
evolution the sequence of the mature protein contains the highly conserved motifs (QXXXGXW/DCTAE) of OSCs and (MWCYCR) of beta-amyrin synthases Eschenbachia blinii
physiological function Conyza blinii is a widely used medicinal herb in southwestern China. The main pharmacological components of Conyza blinii are a class of oleanane-type pentacyclic triterpene glycosides, known as conyzasaponins, which are thought to be synthesized from beta-amyrin. The beta-amyrin synthase mediates cyclization of 2,3-oxidosqualene to yield beta-amyrin. The enzyme is an oxidosqualene cyclase (OSC) involved in conyzasaponin biosynthesis Eschenbachia blinii