BRENDA - Enzyme Database show
show all sequences of 5.4.99.32

Protostadienol synthase from Aspergillus fumigatus: functional conversion into lanosterol synthase

Kimura, M.; Kushiro, T.; Shibuya, M.; Ebizuka, Y.; Abe, I.; Biochem. Biophys. Res. Commun. 391, 899-902 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain GIL77
Aspergillus fumigatus
Engineering
Amino acid exchange
Commentary
Organism
additional information
an OSPC mutant in which the C-terminal residues 702APPGGMR708 are replaced with 702NKSCAIS708, no longer produces the protostadienol, but instead efficiently produces a 1:1 mixture of lanosterol and parkeol (oxidosqualene:lanosterol cyclase activity)
Aspergillus fumigatus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Aspergillus fumigatus
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
83090
-
calculated from amino acid sequence
Aspergillus fumigatus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-oxidosqualene
-
714021
Aspergillus fumigatus
protosta-17(20)Z,24-dien-3beta-ol + (20R)-protosta-13(17),24-dien-3beta-ol
3:1 product ratio
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain GIL77
Aspergillus fumigatus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
an OSPC mutant in which the C-terminal residues 702APPGGMR708 are replaced with 702NKSCAIS708, no longer produces the protostadienol, but instead efficiently produces a 1:1 mixture of lanosterol and parkeol (oxidosqualene:lanosterol cyclase activity)
Aspergillus fumigatus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Aspergillus fumigatus
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
83090
-
calculated from amino acid sequence
Aspergillus fumigatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-oxidosqualene
-
714021
Aspergillus fumigatus
protosta-17(20)Z,24-dien-3beta-ol + (20R)-protosta-13(17),24-dien-3beta-ol
3:1 product ratio
-
-
?
Other publictions for EC 5.4.99.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714021
Kimura
Protostadienol synthase from A ...
Aspergillus fumigatus
Biochem. Biophys. Res. Commun.
391
899-902
2010
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716417
Lodeiro
Protostadienol biosynthesis an ...
Aspergillus fumigatus, Aspergillus fumigatus Af293
Org. Lett.
11
1241-1244
2009
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