Crystallization (Comment) | Organism |
---|---|
the TruB crystallization construct (tTruB), differs from full-length Escherichia coli TruB in having an N-terminal His-tag followed by residue 10 of the full-length protein. tTruB(D48N) is cocrystallized with [5-fluorouridine]-RNA. Initially, crystals are obtained by seeding with fragments of tTruBRNA cocrystals. The resulting crystals are crushed and used as seeds for six subsequent sequential rounds of seeding into tTruB(D48N)RNA complex crystallization experiments | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D48N | inactive mutant enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
tRNA uridine55 | pseudouridine synthase TruB with an 10-amino acid N-terminal truncation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P60340 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA uridine55 | - |
Escherichia coli | tRNA pseudouridine55 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pseudouridine synthase TruB | - |
Escherichia coli |
PSI synthase TruB | - |
Escherichia coli |
TruB | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.78 | - |
tRNA uridine55 | pseudouridine synthase TruB with an 10-amino acid N-terminal truncation | Escherichia coli |