BRENDA - Enzyme Database show
show all sequences of 5.4.99.20

Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli

Del Campo, M.; Kaya, Y.; Ofengand, J.; RNA 7, 1603-1615 (2001)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D79N
inactive mutant enzyme
Escherichia coli
D79T
inactive mutant enzyme
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P75966
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-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
23S rRNA uridine2457
the enzyme has an essential Asp at postion 79
706734
Escherichia coli
23S rRNA pseudouridine2457
-
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D79N
inactive mutant enzyme
Escherichia coli
D79T
inactive mutant enzyme
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
23S rRNA uridine2457
the enzyme has an essential Asp at postion 79
706734
Escherichia coli
23S rRNA pseudouridine2457
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
pseudouridine2457 is absent in a ymfC deletion strain, it reappears when the deletion strain is transformed by a rescue plasmid carrying the ymfC structural gene
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
pseudouridine2457 is absent in a ymfC deletion strain, it reappears when the deletion strain is transformed by a rescue plasmid carrying the ymfC structural gene
Escherichia coli
Other publictions for EC 5.4.99.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729920
Kim
Transcriptional organization a ...
Streptococcus mutans, Streptococcus mutans UA159
J. Bacteriol.
194
1968-1978
2012
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15
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-
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-
-
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1
1
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681422
Pan
The crystal structure of E. co ...
Escherichia coli
J. Mol. Biol.
367
1459-1470
2007
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1
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1
1
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1
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2
1
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1
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1
1
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2
1
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706734
Del Campo
Identification and site of act ...
Escherichia coli
RNA
7
1603-1615
2001
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2
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1
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2
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1
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1
1
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