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Literature summary for 5.4.99.11 extracted from
- Purification and characterization of a highly selective sucrose isomerase from Erwinia rhapontici NX-5 (2011), Bioprocess Biosyst. Eng., 34, 629-637.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 8.3% residual activity at 5 mM | Erwinia rhapontici |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 222 | - |
sucrose | in 25 mM PBS (pH 5.8), at 30°C | Erwinia rhapontici | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 109.5% activity at 5 mM | Erwinia rhapontici | |
| Mn2+ | 119.1% activity at 5 mM | Erwinia rhapontici | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
1 * 65000, SDS-PAGE | Erwinia rhapontici |
| 65600 | - |
gel filtration | Erwinia rhapontici |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Erwinia rhapontici | - |
- |
- |
| Erwinia rhapontici NX-5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephacryl S-300 gel filtration | Erwinia rhapontici |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | - |
crude extract, in 25 mM PBS (pH 5.8), at 30°C | Erwinia rhapontici |
| 427.1 | - |
after 213.6fold purification, in 25 mM PBS (pH 5.8), at 30°C | Erwinia rhapontici |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 30°C, purified enzyme in 25 mM PBS (pH 5.8), 2 weeks, 35% loss of activity | Erwinia rhapontici |
| 4°C, purified enzyme in 25 mM PBS (pH 5.8), 2 weeks, 23% loss of activity | Erwinia rhapontici |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose | - |
Erwinia rhapontici | isomaltulose + trehalulose | - |
? | |
| sucrose | - |
Erwinia rhapontici NX-5 | isomaltulose + trehalulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 65000, SDS-PAGE | Erwinia rhapontici |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SIase | - |
Erwinia rhapontici |
| sucrose isomerase | - |
Erwinia rhapontici |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Erwinia rhapontici |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | more than 70% of the initial activity remaining between 20 and 50°C | Erwinia rhapontici |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
no activity at 60°C after 30 min | Erwinia rhapontici |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Erwinia rhapontici |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | the activity diminishes significantly at a pH above 9.0 or below 5.0, and completely inactivates at pH 10.0 | Erwinia rhapontici |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | the enzyme retains more than 60% of its original activity over a range of pH 5.0-7.0 at 30°C for 24 h | Erwinia rhapontici |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Erwinia rhapontici | isoelectric focusing | - |
6 |
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