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Literature summary for 5.4.99.11 extracted from

  • Cha, J.; Jung, J.H.; Park, S.E.; Cho, M.H.; Seo, D.H.; Ha, S.J.; Yoon, J.W.; Lee, O.H.; Kim, Y.C.; Park, C.S.
    Molecular cloning and functional characterization of a sucrose isomerase (isomaltulose synthase) gene from Enterobacter sp. FMB-1 (2009), J. Appl. Microbiol., 107, 1119-1130.
    View publication on PubMed
Search for more literature for 5.4.99.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Enterobacter sp. FMB-1

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 91% residual activity at 5 mM Enterobacter sp. FMB-1
Mg2+ 98% residual activity at 5 mM Enterobacter sp. FMB-1
Zn2+ 96% residual activity at 5 mM Enterobacter sp. FMB-1

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 115% relative activity at 5 mM Enterobacter sp. FMB-1
EDTA 119% relative activity at 5 mM Enterobacter sp. FMB-1
Fe2+ 126% relative activity at 5 mM Enterobacter sp. FMB-1
Mn2+ 164% relative activity at 5 mM Enterobacter sp. FMB-1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 SDS-PAGE Enterobacter sp. FMB-1
70180
-
 calculated from amino acid sequence Enterobacter sp. FMB-1

Organism

Organism UniProt Comment Textmining
Enterobacter sp. FMB-1 B5ABD8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, gel filtration Enterobacter sp. FMB-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose
-
 Enterobacter sp. FMB-1 isomaltulose + trehalulose the product ratio is 78% isomaltulose and 22% trehalulose ?

Synonyms

Synonyms Comment Organism
isomaltulose synthase
-
 Enterobacter sp. FMB-1
SIase
-
 Enterobacter sp. FMB-1
sucrose isomerase
-
 Enterobacter sp. FMB-1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Enterobacter sp. FMB-1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 70 about 50% activity at 20°C, about 80% activity at 30°C, 100% activity at 40-50°C, about 20% activity at 55°C, complete loss of activity at 60-70°C Enterobacter sp. FMB-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Enterobacter sp. FMB-1

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 the enzymatic activity is significantly lower outside the optimal pH range of 5.0-6.0 implying that the enzyme is very sensitive to pH Enterobacter sp. FMB-1

pI Value

Organism Comment pI Value Maximum pI Value
Enterobacter sp. FMB-1 calculated from amino acid sequence
-
 6