Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-D-Glucose 1,6-bisphosphate | about 7fold increase of activity at 0.001 mM | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
General Stability | Organism |
---|---|
using 10% (v/v) glycerol, the enzyme maintains 86% of its initial activity at pH 10.0 after 18 h incubation | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | highest activity at 0.1 mM | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-ribose 1-phosphate | Escherichia coli | - |
D-ribose 5-phosphate | - |
? | |
alpha-D-ribose 1-phosphate | Escherichia coli ATCC 4157 | - |
D-ribose 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli ATCC 4157 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-ribose 1-phosphate | - |
Escherichia coli | D-ribose 5-phosphate | - |
? | |
alpha-D-ribose 1-phosphate | - |
Escherichia coli ATCC 4157 | D-ribose 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PPM | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
enzyme immobilized on CNBr-Ag is completely inactive after 16 h incubation at 50°C. Enzymes immobilized on cyanogen bromide or glyoxyl agarose maintain 100% of their initial activity after 16 h at 50°C. Enzyme immobilized on monoaminoethyl-N-aminoethyl-glutaraldehyde or monoaminoethyl-N-aminoethyl-agarose loses more than 50% of its initial activity after 16 h at 50°C | Escherichia coli |