Crystallization (Comment) | Organism |
---|---|
phospho- and dephospho-enzyme in complex with reaction intermediate glucose 1,6-bisphosphate at 1.9 and 2.0 A | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
N110A | no remarkable differences in Km and Vmax value compared to wild-type, but intermediate glucose-1,6-bisphosphate dissociates from mutant 25times more often than from wild-type | Pseudomonas aeruginosa |
R15A | no remarkable differences in Km and Vmax value compared to wild-type, but intermediate glucose-1,6-bisphosphate dissociates from mutant 25times more often than from wild-type | Pseudomonas aeruginosa |
R20A | no catalytic activity | Pseudomonas aeruginosa |
R241C | 0.3% of wild-type acivity, with Km value similar to wild-type | Pseudomonas aeruginosa |
R247A | no remarkable differences in Km and Vmax value compared to wild-type, modest increase in dissociation of intermediate glucose-1,6-bisphosphate from enzyme | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0127 | - |
alpha-D-glucose 1-phosphate | mutant RN110A | Pseudomonas aeruginosa | |
0.0132 | - |
alpha-D-glucose 1-phosphate | mutant R15A | Pseudomonas aeruginosa | |
0.0195 | - |
alpha-D-glucose 1-phosphate | mutant R247A | Pseudomonas aeruginosa | |
0.0273 | - |
alpha-D-glucose 1-phosphate | wild-type | Pseudomonas aeruginosa | |
0.0279 | - |
alpha-D-glucose 1-phosphate | mutant R421C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
bifunctional phosphomannomutase/phosphoglucomutase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | - |
Pseudomonas aeruginosa | alpha-D-glucose 6-phosphate | - |
? |