Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | independent on 2,3-bisphosphoglycerate | Dirofilaria immitis | |
additional information | independent on 2,3-bisphosphoglycerate | Wolbachia sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C2566/T7 cells | Wolbachia endosymbiont of Dirofilaria immitis |
expressed in Escherichia coli C2566/T7 cells | Dirofilaria immitis |
expression of C-terminally His-tagged enzyme in Escherichia coli strain C2566/T7 | Wolbachia sp. |
expression of C-terminally His6-tagged enzyme in Escherichia coli strain C2566/T7 | Dirofilaria immitis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Dirofilaria immitis | |
additional information | - |
additional information | Michaelis-Menten kinetics | Wolbachia sp. | |
0.55 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Wolbachia sp. | |
0.55 | - |
3-phospho-D-glycerate | in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Wolbachia endosymbiont of Dirofilaria immitis | |
0.58 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Dirofilaria immitis | |
0.58 | - |
3-phospho-D-glycerate | in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Dirofilaria immitis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, calculated from amino acid sequence | Wolbachia endosymbiont of Dirofilaria immitis |
57000 | - |
x * 57000, calculated from amino acid sequence | Dirofilaria immitis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Dirofilaria immitis | - |
3-phospho-D-glycerate | - |
r | |
2-phospho-D-glycerate | Wolbachia sp. | - |
3-phospho-D-glycerate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dirofilaria immitis | - |
- |
- |
Dirofilaria immitis | F4ZE01 | - |
- |
Wolbachia endosymbiont of Dirofilaria immitis | F4ZE02 | - |
- |
Wolbachia sp. | - |
endosymbiont of filarial parasite Brugia malayi | - |
Purification (Comment) | Organism |
---|---|
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography | Wolbachia endosymbiont of Dirofilaria immitis |
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography | Dirofilaria immitis |
recombinant His6-tagged enzyme from Escherichia coli strain C2566/T7 by nicke affinity chromatography, dialysis and anion exchange chromatography | Wolbachia sp. |
recombinant His6-tagged enzyme from Escherichia coli strain C2566/T7 by nickel affinity chromatography, dialysis and anion exchange chromatography | Dirofilaria immitis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimal enzyme production at 37°C | Wolbachia sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
71 | - |
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Wolbachia endosymbiont of Dirofilaria immitis |
71 | - |
purified recombinant His-tagged enzyme, pH 7.5, 30°C | Wolbachia sp. |
225 | - |
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Dirofilaria immitis |
225 | - |
purified recombinant His-tagged enzyme, pH 7.5, 30°C | Dirofilaria immitis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Dirofilaria immitis | 3-phospho-D-glycerate | - |
r | |
2-phospho-D-glycerate | - |
Wolbachia sp. | 3-phospho-D-glycerate | - |
r | |
2-phospho-D-glycerate | - |
Wolbachia endosymbiont of Dirofilaria immitis | 3-phospho-D-glycerate | - |
r | |
3-phospho-D-glycerate | - |
Dirofilaria immitis | 2-phospho-D-glycerate | - |
r | |
3-phospho-D-glycerate | - |
Wolbachia sp. | 2-phospho-D-glycerate | - |
r | |
3-phospho-D-glycerate | - |
Wolbachia endosymbiont of Dirofilaria immitis | 2-phospho-D-glycerate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, calculated from amino acid sequence | Wolbachia endosymbiont of Dirofilaria immitis |
? | x * 57000, calculated from amino acid sequence | Dirofilaria immitis |
Synonyms | Comment | Organism |
---|---|---|
cofactor-independent phosphoglycerate mutase | - |
Dirofilaria immitis |
cofactor-independent phosphoglycerate mutase | - |
Wolbachia sp. |
independent PGM | the enzyme requires 2,3-bisphosphoglycerate as a cofactor | Wolbachia endosymbiont of Dirofilaria immitis |
independent PGM | the enzyme requires 2,3-bisphosphoglycerate as a cofactor | Dirofilaria immitis |
iPGM | - |
Dirofilaria immitis |
iPGM | - |
Wolbachia sp. |
iPGM | the enzyme requires 2,3-bisphosphoglycerate as a cofactor | Wolbachia endosymbiont of Dirofilaria immitis |
iPGM | the enzyme requires 2,3-bisphosphoglycerate as a cofactor | Dirofilaria immitis |
PGM | - |
Wolbachia endosymbiont of Dirofilaria immitis |
PGM | - |
Dirofilaria immitis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Dirofilaria immitis |
30 | - |
assay at | Wolbachia sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
102 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Wolbachia sp. | |
102 | - |
3-phospho-D-glycerate | in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Wolbachia endosymbiont of Dirofilaria immitis | |
132 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Dirofilaria immitis | |
132 | - |
3-phospho-D-glycerate | in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C | Dirofilaria immitis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Dirofilaria immitis |
7.5 | - |
assay at | Wolbachia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | 2,3-bisphosphoglycerate is not required as a cofactor | Wolbachia endosymbiont of Dirofilaria immitis | |
additional information | 2,3-bisphosphoglycerate is not required as a cofactor | Dirofilaria immitis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Dirofilaria immitis | calculated from amino acid sequence | - |
6.3 |
Wolbachia endosymbiont of Dirofilaria immitis | calculated from amino acid sequence | - |
7.4 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
186 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Wolbachia sp. | |
228 | - |
3-phospho-D-glycerate | recombinant His-tagged enzyme, pH 7.5, 30°C | Dirofilaria immitis |