Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli with C-terminal His-tag | Leishmania mexicana |
General Stability | Organism |
---|---|
rapid decrease in activity when no stabilizer is added. In presence of NaCl, CoCl2, and imidazole, stable for 1 month at 4°C | Leishmania mexicana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | irreversible inhibition, saturating concentrations of substrate protect | Leishmania mexicana | |
EDTA | 1 mM, 10% residual activity at pH 8.0, reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4, NiCl2, ZnCl2 | Leishmania mexicana | |
additional information | not inhibitory: vanadate, cloride, sulfate | Leishmania mexicana | |
phosphate | 100 mM, 70% residual activity | Leishmania mexicana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
2-phospho-D-glycerate | pH 7.6, 25°C | Leishmania mexicana | |
0.27 | - |
3-phospho-D-glycerate | pH 7.6, 25°C | Leishmania mexicana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Leishmania mexicana | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | inhibition by EDTA is reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4, NiCl2, ZnCl2 | Leishmania mexicana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE | Leishmania mexicana |
60724 | - |
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE | Leishmania mexicana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania mexicana | Q86N96 | sp. mexicana | - |
Renatured (Comment) | Organism |
---|---|
inactivation by EDTA is reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4,NiCl2, ZnCl2 | Leishmania mexicana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
419 | - |
formation of 2-phospho-D-glycerate, pH 7.6, 25°C | Leishmania mexicana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Leishmania mexicana | 3-phospho-D-glycerate | - |
r | |
3-phospho-D-glycerate | - |
Leishmania mexicana | 2-phospho-D-glycerate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE | Leishmania mexicana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
199 | - |
2-phospho-D-glycerate | pH 7.6, 25°C | Leishmania mexicana | |
434 | - |
3-phospho-D-glycerate | pH 7.6, 25°C | Leishmania mexicana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.2 | for both directions of reaction | Leishmania mexicana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.8 | more than 75% of maximum activity within this range, formation of 2-phospho-D-glycerate | Leishmania mexicana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | 2,3-bisphosphoglycerate independent phosphoglycerate mutase | Leishmania mexicana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leishmania mexicana | calculated | - |
5.26 |