Cloned (Comment) | Organism |
---|---|
expression in Spodoptera frugiperda 21 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C441H | no heme-binding ability, no activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tranylcypromine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
prostaglandin H2 | at 24°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | localization of recombinant enzyme in infected Sf21 cells | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
wild-type, SDS-PAGE | Homo sapiens |
48000 | - |
mutant C441H, SDS-PAGE | Homo sapiens |
57100 | - |
calculated from the primary structure, deduced from the cDNA sequence | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prostaglandin H2 | Homo sapiens | - |
prostacyclin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oxidation Stability | Organism |
---|---|
human PGIS is significantly unstable when the heme cofactor appears in the reduction and/or reduction-CO binding state | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme is purified to homogeneity | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
solubilized microsomes | Homo sapiens |
2.6 | - |
after calcium phosphate gel | Homo sapiens |
4.3 | - |
after DEAE-sepharose chromatography | Homo sapiens |
7.1 | - |
after hydroxyapatite chromatography | Homo sapiens |
15 | - |
purified enzyme, at 24°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prostaglandin H2 | - |
Homo sapiens | prostacyclin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGIS | - |
Homo sapiens |
prostacyclin synthase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Homo sapiens |