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Literature summary for 5.3.99.12 extracted from
- Discovery and characterization of a novel lachrymatory factor synthase in Petiveria alliacea and its influence on alliinase-mediated formation of biologically active organosulfur compounds (2009), Plant Physiol., 151, 1294-1303 .
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 217700 | - |
gel filtration | Petiveria alliacea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phenylmethanesulfenic acid | Petiveria alliacea | the enzyme catalyzes the formation of a sulfine lachrymator, (Z)-phenylmethanethial S-oxide, only in the presence of Petiveria alliacea alliinase and its natural substrate, S-benzyl-L-cysteine sulfoxide (petiveriin) | (Z)-phenylmethanethial S-oxide | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Petiveria alliacea | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the two alpha-subunits are glycosylated and connected by a disulfide bridge | Petiveria alliacea |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE column chromatography, hydroxyapatite column chromatography, and Bio-Sil SEC-250 gel filtration | Petiveria alliacea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Petiveria alliacea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.012 | - |
after 2.2fold purification, at pH 8.0 and 25°C | Petiveria alliacea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phenylmethanesulfenic acid | the enzyme catalyzes the formation of a sulfine lachrymator, (Z)-phenylmethanethial S-oxide, only in the presence of Petiveria alliacea alliinase and its natural substrate, S-benzyl-L-cysteine sulfoxide (petiveriin) | Petiveria alliacea | (Z)-phenylmethanethial S-oxide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | 2 * 688000 + 1 * 22500 + 1 * 11900, calculated from amino acid sequence | Petiveria alliacea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lachrymatory factor synthase | - |
Petiveria alliacea |
| LFS | - |
Petiveria alliacea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Petiveria alliacea | isoelectric focusing | - |
5.2 |
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Release 2023.1 (January 2023)
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