Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
3-trans-hexenoyl-CoA | pH 7.5, 25°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Rattus norvegicus |
60000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | key enzyme for the beta-oxidation of unsaturated fatty acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
201 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, stable for at least 6 months | Rattus norvegicus |
4°C, stable for 2 days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-trans-Hexenoyl-CoA | - |
Rattus norvegicus | 2-trans-Hexenoyl-CoA | - |
? | |
additional information | key enzyme for the beta-oxidation of unsaturated fatty acids | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
8 h, stable at room temperature | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | phosphate buffer | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | at least 60% of maximal activity over the pH range 6 to 8.5 | Rattus norvegicus |