BRENDA - Enzyme Database
show all sequences of 5.3.3.6

Crystal structure and putative mechanism of 3-methylitaconate-DELTA-isomerase from Eubacterium barkeri

Velarde, M.; Macieira, S.; Hilberg, M.; Bröker, G.; Tu, S.-M.; Golding, B.T.; Pierik, A.J.; Buckel, W.; Messerschmidt, A.; J. Mol. Biol. 391, 609-620 (2009) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
into a pASG-IBA3 vector for expression in Escherichia coli Rosetta cells
Eubacterium barkeri
Crystallization (Commentary)
Crystallization (Commentary)
Organism
the crystal structure of 3-methylitaconate-DELTA-isomerase is solved at a resolution of 2.7 A
Eubacterium barkeri
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7
-
methylitaconate
(R,S)-3-methylitaconate
Eubacterium barkeri
60
-
Itaconate
-
Eubacterium barkeri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40270
-
theoretical, without Strep-tag
Eubacterium barkeri
41500
-
theoretical, including the Strep-tag
Eubacterium barkeri
45000
-
apparent molecular mass, determined by SDS-PAGE
Eubacterium barkeri
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
methylitaconate
Eubacterium barkeri
-
2,3-dimethylmaleate
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Eubacterium barkeri
Q0QLE6
-
-
Purification (Commentary)
Purification (Commentary)
Organism
affinity purification on a streptavidin column
Eubacterium barkeri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
itaconate
-
705153
Eubacterium barkeri
methylmaleate
-
-
-
?
methylitaconate
-
705153
Eubacterium barkeri
2,3-dimethylmaleate
-
-
-
r
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 40270 Da
Eubacterium barkeri
Synonyms
Synonyms
Commentary
Organism
3-methylitaconate-DELTA-isomerase
-
Eubacterium barkeri
MII
-
Eubacterium barkeri
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
activity assay
Eubacterium barkeri
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2
-
Itaconate
-
Eubacterium barkeri
1720
-
methylitaconate
(R,S)-3-methylitaconate
Eubacterium barkeri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
activity assay
Eubacterium barkeri
Cloned(Commentary) (protein specific)
Commentary
Organism
into a pASG-IBA3 vector for expression in Escherichia coli Rosetta cells
Eubacterium barkeri
Crystallization (Commentary) (protein specific)
Crystallization
Organism
the crystal structure of 3-methylitaconate-DELTA-isomerase is solved at a resolution of 2.7 A
Eubacterium barkeri
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7
-
methylitaconate
(R,S)-3-methylitaconate
Eubacterium barkeri
60
-
Itaconate
-
Eubacterium barkeri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40270
-
theoretical, without Strep-tag
Eubacterium barkeri
41500
-
theoretical, including the Strep-tag
Eubacterium barkeri
45000
-
apparent molecular mass, determined by SDS-PAGE
Eubacterium barkeri
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
methylitaconate
Eubacterium barkeri
-
2,3-dimethylmaleate
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
affinity purification on a streptavidin column
Eubacterium barkeri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
itaconate
-
705153
Eubacterium barkeri
methylmaleate
-
-
-
?
methylitaconate
-
705153
Eubacterium barkeri
2,3-dimethylmaleate
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 40270 Da
Eubacterium barkeri
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
activity assay
Eubacterium barkeri
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2
-
Itaconate
-
Eubacterium barkeri
1720
-
methylitaconate
(R,S)-3-methylitaconate
Eubacterium barkeri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
activity assay
Eubacterium barkeri
General Information
General Information
Commentary
Organism
metabolism
3-methylitaconate-DELTA-isomerase participates in the nicotinate fermentation pathway
Eubacterium barkeri
General Information (protein specific)
General Information
Commentary
Organism
metabolism
3-methylitaconate-DELTA-isomerase participates in the nicotinate fermentation pathway
Eubacterium barkeri
Other publictions for EC 5.3.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
705153
Velarde
Crystal structure and putative ...
Eubacterium barkeri
J. Mol. Biol.
391
609-620
2009
-
-
1
1
-
-
-
2
-
-
3
1
-
11
-
-
1
-
-
-
-
-
2
1
2
1
-
-
2
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
3
1
-
-
-
1
-
-
-
-
2
1
1
-
-
2
1
-
-
-
-
1
1
-
-
-
670692
Alhapel
Molecular and functional analy ...
Eubacterium barkeri
Proc. Natl. Acad. Sci. USA
103
12341-12346
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2988
Hartrampf
On the steric course of the ad ...
Eubacterium barkeri
Eur. J. Biochem.
156
301-304
1986
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2986
Kung
Nicotinic acid metabolism. VII ...
Eubacterium barkeri
J. Biol. Chem.
246
6436-6443
1971
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3522
Kung
Nicotinic acid metabolism. VI. ...
Eubacterium barkeri
J. Biol. Chem.
246
3378-3388
1971
3
-
-
-
-
-
3
2
-
1
1
-
-
1
-
-
1
-
-
-
1
3
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
3
-
2
-
1
1
-
-
-
-
1
-
-
1
3
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-