BRENDA - Enzyme Database
show all sequences of 5.3.3.6

Nicotinic acid metabolism. VI. Purification and properties of alpha-methyleneglutarate mutase (B12-dependent) and methylitaconate isomerase

Kung, H.F.; Stadtman, T.C.; J. Biol. Chem. 246, 3378-3388 (1971) View publication on PubMed

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
10 mM, 30% increase in activity
Eubacterium barkeri
mercaptan
required for optimal activity
Eubacterium barkeri
additional information
enzyme contains sulfhydryl groups
Eubacterium barkeri
Inhibitors
Inhibitors
Commentary
Organism
Structure
GSSG
-
Eubacterium barkeri
iodoacetic acid
-
Eubacterium barkeri
p-chloromercuriphenyl sulfonic acid
-
Eubacterium barkeri
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4
-
dimethylmaleate
-
Eubacterium barkeri
6.2
-
methylitaconate
-
Eubacterium barkeri
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ion required
Eubacterium barkeri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
140000
-
sucrose density gradient centrifugation
Eubacterium barkeri
Organism
Organism
UniProt
Commentary
Textmining
Eubacterium barkeri
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Eubacterium barkeri
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Eubacterium barkeri
Storage Stability
Storage Stability
Organism
-20°C, protein concentration 1 mg/ml, little loss of activity after 60 days
Eubacterium barkeri
-80°C, protein concentration 10 mg/ml, less than 10% loss of activity after 6 months
Eubacterium barkeri
0°C, protein concentration 1 mg/ml, little loss of activity after 6 days
Eubacterium barkeri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Methylitaconate
r
3522
Eubacterium barkeri
Dimethylmaleate
-
3522
Eubacterium barkeri
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
8.2
-
Eubacterium barkeri
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
10 mM, 30% increase in activity
Eubacterium barkeri
mercaptan
required for optimal activity
Eubacterium barkeri
additional information
enzyme contains sulfhydryl groups
Eubacterium barkeri
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
GSSG
-
Eubacterium barkeri
iodoacetic acid
-
Eubacterium barkeri
p-chloromercuriphenyl sulfonic acid
-
Eubacterium barkeri
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4
-
dimethylmaleate
-
Eubacterium barkeri
6.2
-
methylitaconate
-
Eubacterium barkeri
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ion required
Eubacterium barkeri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
140000
-
sucrose density gradient centrifugation
Eubacterium barkeri
Purification (Commentary) (protein specific)
Commentary
Organism
-
Eubacterium barkeri
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Eubacterium barkeri
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, protein concentration 1 mg/ml, little loss of activity after 60 days
Eubacterium barkeri
-80°C, protein concentration 10 mg/ml, less than 10% loss of activity after 6 months
Eubacterium barkeri
0°C, protein concentration 1 mg/ml, little loss of activity after 6 days
Eubacterium barkeri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Methylitaconate
r
3522
Eubacterium barkeri
Dimethylmaleate
-
3522
Eubacterium barkeri
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
8.2
-
Eubacterium barkeri
Other publictions for EC 5.3.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
705153
Velarde
Crystal structure and putative ...
Eubacterium barkeri
J. Mol. Biol.
391
609-620
2009
-
-
1
1
-
-
-
2
-
-
3
1
-
11
-
-
1
-
-
-
-
-
2
1
2
1
-
-
2
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
3
1
-
-
-
1
-
-
-
-
2
1
1
-
-
2
1
-
-
-
-
1
1
-
-
-
670692
Alhapel
Molecular and functional analy ...
Eubacterium barkeri
Proc. Natl. Acad. Sci. USA
103
12341-12346
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
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-
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-
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2988
Hartrampf
On the steric course of the ad ...
Eubacterium barkeri
Eur. J. Biochem.
156
301-304
1986
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-
-
-
-
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-
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-
-
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-
1
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1
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
2986
Kung
Nicotinic acid metabolism. VII ...
Eubacterium barkeri
J. Biol. Chem.
246
6436-6443
1971
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-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
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1
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3
-
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1
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1
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3
-
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-
-
-
-
-
-
3522
Kung
Nicotinic acid metabolism. VI. ...
Eubacterium barkeri
J. Biol. Chem.
246
3378-3388
1971
3
-
-
-
-
-
3
2
-
1
1
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1
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1
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1
3
1
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1
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3
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3
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2
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1
1
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1
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1
3
1
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1
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