Nicotinic acid metabolism. VI. Purification and properties of alpha-methyleneglutarate mutase (B12-dependent) and methylitaconate isomerase

Kung, H.F.; Stadtman, T.C.; J. Biol. Chem. 246, 3378-3388 (1971)

Data extracted from this reference:

Activating Compound

Commentary

Organism

Structure

EDTA

10 mM, 30% increase in activity

Eubacterium barkeri

mercaptan

required for optimal activity

Eubacterium barkeri

additional information

enzyme contains sulfhydryl groups

Eubacterium barkeri

Inhibitors

Commentary

Organism

Structure

GSSG

-

Eubacterium barkeri

iodoacetic acid

-

Eubacterium barkeri

p-chloromercuriphenyl sulfonic acid

-

Eubacterium barkeri

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

4

-

dimethylmaleate

-

Eubacterium barkeri

6.2

-

methylitaconate

-

Eubacterium barkeri

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

Methylitaconate

r

3522

Eubacterium barkeri

Dimethylmaleate

-

3522

Eubacterium barkeri

?

Activating Compound

Commentary

Organism

Structure

EDTA

10 mM, 30% increase in activity

Eubacterium barkeri

mercaptan

required for optimal activity

Eubacterium barkeri

additional information

enzyme contains sulfhydryl groups

Eubacterium barkeri

Inhibitors

Commentary

Organism

Structure

GSSG

-

Eubacterium barkeri

iodoacetic acid

-

Eubacterium barkeri

p-chloromercuriphenyl sulfonic acid

-

Eubacterium barkeri

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

4

-

dimethylmaleate

-

Eubacterium barkeri

6.2

-

methylitaconate

-

Eubacterium barkeri

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

Methylitaconate

r

3522

Eubacterium barkeri

Dimethylmaleate

-

3522

Eubacterium barkeri

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

705153

Velarde

Crystal structure and putative ...

Eubacterium barkeri

J. Mol. Biol.

391

609-620

2009

-

-

1

1

-

-

-

2

-

-

3

1

-

11

-

-

1

-

-

-

-

-

2

1

2

1

-

-

2

1

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

2

-

-

3

1

-

-

-

1

-

-

-

-

2

1

1

-

-

2

1

-

-

-

-

1

1

-

-

-

670692

Alhapel

Molecular and functional analy ...

Eubacterium barkeri

Proc. Natl. Acad. Sci. USA

103

12341-12346

2006

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2988

Hartrampf

On the steric course of the ad ...

Eubacterium barkeri

Eur. J. Biochem.

156

301-304

1986

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2986

Kung

Nicotinic acid metabolism. VII ...

Eubacterium barkeri

J. Biol. Chem.

246

6436-6443

1971

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

1

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

1

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

3522

Kung

Nicotinic acid metabolism. VI. ...

Eubacterium barkeri

J. Biol. Chem.

246

3378-3388

1971

3

-

-

-

-

-

3

2

-

1

1

-

-

1

-

-

1

-

-

-

1

3

1

-

-

-

-

-

-

1

-

-

-

-

-

-

3

-

-

-

-

-

-

-

3

-

2

-

1

1

-

-

-

-

1

-

-

1

3

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-