BRENDA - Enzyme Database
show all sequences of 5.3.3.5

Histidine77, glutamic acid81, glutamic acid123, threonine126, asparagine194, and tryptophan197 of the human emopamil binding protein are required for in vivo sterol DELTA8-DELTA7 isomerization

Moebius, F.F.; Soellner, K.E.M.; Fiechtner, B.; Huck, C.W.; Bonn, G.; Glossmann, H.; Biochemistry 38, 1119-1127 (1999)

Data extracted from this reference:

Engineering
Protein Variants
Commentary
Organism
C121A
mutant enzyme with 95% of the in vivo activity of the wild-type enzyme
Homo sapiens
C128A
mutant enzyme with 81% of the in vivo activity of the wild-type enzyme
Homo sapiens
C68A
mutant enzyme with 94% of the in vivo activity of the wild-type enzyme
Homo sapiens
C73A
mutant enzyme with 83% of the in vivo activity of the wild-type enzyme
Homo sapiens
E123A
mutant enzyme with 10% of the in vivo activity of the wild-type enzyme
Homo sapiens
E123D
mutant enzyme does not bind the inhibitor ifenprodil
Homo sapiens
E81A
mutant enzyme with 5% of the in vivo activity of the wild-type enzyme
Homo sapiens
E81D
mutant enzyme still binds the inhibitor ifenprodil
Homo sapiens
F186A
mutant enzyme with 56% of the in vivo activity of the wild-type enzyme
Homo sapiens
F188A
mutant enzyme with 94% of the in vivo activity of the wild-type enzyme
Homo sapiens
F190A
mutant enzyme with 21% of the in vivo activity of the wild-type enzyme
Homo sapiens
F192A
mutant enzyme with 92% of the in vivo activity of the wild-type enzyme
Homo sapiens
F37A
mutant enzyme with 74% of the in vivo activity of the wild-type enzyme
Homo sapiens
F70A
mutant enzyme with 95% of the in vivo activity of the wild-type enzyme
Homo sapiens
F75A
mutant enzyme with 88% of the in vivo activity of the wild-type enzyme
Homo sapiens
F84A
mutant enzyme with 72% of the in vivo activity of the wild-type enzyme
Homo sapiens
G131A
mutant enzyme with 69% of the in vivo activity of the wild-type enzyme
Homo sapiens
G41A
mutant enzyme with 94% of the in vivo activity of the wild-type enzyme
Homo sapiens
G82A/F84V
mutant enzyme with 56% of the in vivo activity of the wild-type enzyme
Homo sapiens
H31A
mutant enzyme with 69% of the in vivo activity of the wild-type enzyme
Homo sapiens
H77A
mutant enzyme with 9% of the in vivo activity of the wild-type enzyme
Homo sapiens
H77N
mutant enzyme does not bind the inhibitor ifenprodil
Homo sapiens
I125A
mutant enzyme with 78% of the in vivo activity of the wild-type enzyme
Homo sapiens
I139A
mutant enzyme with 90% of the in vivo activity of the wild-type enzyme
Homo sapiens
I32A
mutant enzyme with 73% of the in vivo activity of the wild-type enzyme
Homo sapiens
I76A
mutant enzyme with 33% of the in vivo activity of the wild-type enzyme
Homo sapiens
I76L/L78A
mutant enzyme with 74% of the in vivo activity of the wild-type enzyme
Homo sapiens
L129A
mutant enzyme with 81% of the in vivo activity of the wild-type enzyme
Homo sapiens
L133A
mutant enzyme with 77% of the in vivo activity of the wild-type enzyme
Homo sapiens
L135A
mutant enzyme with 92% of the in vivo activity of the wild-type enzyme
Homo sapiens
L196A
mutant enzyme with 62% of the in vivo activity of the wild-type enzyme
Homo sapiens
L198A
mutant enzyme with 50% of the in vivo activity of the wild-type enzyme
Homo sapiens
L204A
mutant enzyme with 77% of the in vivo activity of the wild-type enzyme
Homo sapiens
L33A
mutant enzyme with 47% of the in vivo activity of the wild-type enzyme
Homo sapiens
L35A
mutant enzyme with 62% of the in vivo activity of the wild-type enzyme
Homo sapiens
L43A
mutant enzyme with 103% of the in vivo activity of the wild-type enzyme
Homo sapiens
L49A
mutant enzyme with 108% of the in vivo activity of the wild-type enzyme
Homo sapiens
L50A
mutant enzyme with 96% of the in vivo activity of the wild-type enzyme
Homo sapiens
L65A
mutant enzyme with 82% of the in vivo activity of the wild-type enzyme
Homo sapiens
L67A
mutant enzyme with 63% of the in vivo activity of the wild-type enzyme
Homo sapiens
M122A
mutant enzyme with 28% of the in vivo activity of the wild-type enzyme
Homo sapiens
M193A
mutant enzyme with 53% of the in vivo activity of the wild-type enzyme
Homo sapiens
N194Q
mutant enzyme still binds the inhibitor ifenprodil
Homo sapiens
S134A
mutant enzyme with 101% of the in vivo activity of the wild-type enzyme
Homo sapiens
S38A
mutant enzyme with 74% of the in vivo activity of the wild-type enzyme
Homo sapiens
T124A
mutant enzyme with 91% of the in vivo activity of the wild-type enzyme
Homo sapiens
T126A
mutant enzyme with 4% of the in vivo activity of the wild-type enzyme
Homo sapiens
T126S
mutant enzyme still binds the inhibitor ifenprodil
Homo sapiens
V120A
mutant enzyme with 62% of the in vivo activity of the wild-type enzyme
Homo sapiens
V138A
mutant enzyme with 94% of the in vivo activity of the wild-type enzyme
Homo sapiens
V191A
mutant enzyme with 91% of the in vivo activity of the wild-type enzyme
Homo sapiens
V199A
mutant enzyme with 76% of the in vivo activity of the wild-type enzyme
Homo sapiens
V203A
mutant enzyme with 71% of the in vivo activity of the wild-type enzyme
Homo sapiens
V39A
mutant enzyme with 80% of the in vivo activity of the wild-type enzyme
Homo sapiens
V44A
mutant enzyme with 76% of the in vivo activity of the wild-type enzyme
Homo sapiens
V45A
mutant enzyme with 79% of the in vivo activity of the wild-type enzyme
Homo sapiens
V72A
mutant enzyme with 93% of the in vivo activity of the wild-type enzyme
Homo sapiens
V79A
mutant enzyme with 75% of the in vivo activity of the wild-type enzyme
Homo sapiens
V85A
mutant enzyme with 68% of the in vivo activity of the wild-type enzyme
Homo sapiens
W130A
mutant enzyme with 70% of the in vivo activity of the wild-type enzyme
Homo sapiens
W136A
mutant enzyme with 104% of the in vivo activity of the wild-type enzyme
Homo sapiens
W187A
mutant enzyme with 56% of the in vivo activity of the wild-type enzyme
Homo sapiens
W197A
mutant enzyme with 5% of the in vivo activity of the wild-type enzyme
Homo sapiens
W197F
mutant enzyme still binds the inhibitor ifenprodil
Homo sapiens
W48A
mutant enzyme with 87% of the in vivo activity of the wild-type enzyme
Homo sapiens
W69A
mutant enzyme with 17% of the in vivo activity of the wild-type enzyme
Homo sapiens
W83A
mutant enzyme with 97% of the in vivo activity of the wild-type enzyme
Homo sapiens
Y185A
mutant enzyme with 64% of the in vivo activity of the wild-type enzyme
Homo sapiens
Y189A
mutant enzyme with 32% of the in vivo activity of the wild-type enzyme
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
ifenprodil
-
Homo sapiens
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
Q15125
-
-
Synonyms
Synonyms
Commentary
Organism
EBP
-
Homo sapiens
Emopamil binding protein
-
Homo sapiens
Engineering (protein specific)
Protein Variants
Commentary
Organism
C121A
mutant enzyme with 95% of the in vivo activity of the wild-type enzyme
Homo sapiens
C128A
mutant enzyme with 81% of the in vivo activity of the wild-type enzyme
Homo sapiens
C68A
mutant enzyme with 94% of the in vivo activity of the wild-type enzyme
Homo sapiens
C73A
mutant enzyme with 83% of the in vivo activity of the wild-type enzyme
Homo sapiens
E123A
mutant enzyme with 10% of the in vivo activity of the wild-type enzyme
Homo sapiens
E123D
mutant enzyme does not bind the inhibitor ifenprodil
Homo sapiens
E81A
mutant enzyme with 5% of the in vivo activity of the wild-type enzyme
Homo sapiens
E81D
mutant enzyme still binds the inhibitor ifenprodil
Homo sapiens
F186A
mutant enzyme with 56% of the in vivo activity of the wild-type enzyme
Homo sapiens
F188A
mutant enzyme with 94% of the in vivo activity of the wild-type enzyme
Homo sapiens
F190A
mutant enzyme with 21% of the in vivo activity of the wild-type enzyme
Homo sapiens
F192A
mutant enzyme with 92% of the in vivo activity of the wild-type enzyme
Homo sapiens
F37A
mutant enzyme with 74% of the in vivo activity of the wild-type enzyme
Homo sapiens
F70A
mutant enzyme with 95% of the in vivo activity of the wild-type enzyme
Homo sapiens
F75A
mutant enzyme with 88% of the in vivo activity of the wild-type enzyme
Homo sapiens
F84A
mutant enzyme with 72% of the in vivo activity of the wild-type enzyme
Homo sapiens
G131A
mutant enzyme with 69% of the in vivo activity of the wild-type enzyme
Homo sapiens
G41A
mutant enzyme with 94% of the in vivo activity of the wild-type enzyme
Homo sapiens
G82A/F84V
mutant enzyme with 56% of the in vivo activity of the wild-type enzyme
Homo sapiens
H31A
mutant enzyme with 69% of the in vivo activity of the wild-type enzyme
Homo sapiens
H77A
mutant enzyme with 9% of the in vivo activity of the wild-type enzyme
Homo sapiens
H77N
mutant enzyme does not bind the inhibitor ifenprodil
Homo sapiens
I125A
mutant enzyme with 78% of the in vivo activity of the wild-type enzyme
Homo sapiens
I139A
mutant enzyme with 90% of the in vivo activity of the wild-type enzyme
Homo sapiens
I32A
mutant enzyme with 73% of the in vivo activity of the wild-type enzyme
Homo sapiens
I76A
mutant enzyme with 33% of the in vivo activity of the wild-type enzyme
Homo sapiens
I76L/L78A
mutant enzyme with 74% of the in vivo activity of the wild-type enzyme
Homo sapiens
L129A
mutant enzyme with 81% of the in vivo activity of the wild-type enzyme
Homo sapiens
L133A
mutant enzyme with 77% of the in vivo activity of the wild-type enzyme
Homo sapiens
L135A
mutant enzyme with 92% of the in vivo activity of the wild-type enzyme
Homo sapiens
L196A
mutant enzyme with 62% of the in vivo activity of the wild-type enzyme
Homo sapiens
L198A
mutant enzyme with 50% of the in vivo activity of the wild-type enzyme
Homo sapiens
L204A
mutant enzyme with 77% of the in vivo activity of the wild-type enzyme
Homo sapiens
L33A
mutant enzyme with 47% of the in vivo activity of the wild-type enzyme
Homo sapiens
L35A
mutant enzyme with 62% of the in vivo activity of the wild-type enzyme
Homo sapiens
L43A
mutant enzyme with 103% of the in vivo activity of the wild-type enzyme
Homo sapiens
L49A
mutant enzyme with 108% of the in vivo activity of the wild-type enzyme
Homo sapiens
L50A
mutant enzyme with 96% of the in vivo activity of the wild-type enzyme
Homo sapiens
L65A
mutant enzyme with 82% of the in vivo activity of the wild-type enzyme
Homo sapiens
L67A
mutant enzyme with 63% of the in vivo activity of the wild-type enzyme
Homo sapiens
M122A
mutant enzyme with 28% of the in vivo activity of the wild-type enzyme
Homo sapiens
M193A
mutant enzyme with 53% of the in vivo activity of the wild-type enzyme
Homo sapiens
N194Q
mutant enzyme still binds the inhibitor ifenprodil
Homo sapiens
S134A
mutant enzyme with 101% of the in vivo activity of the wild-type enzyme
Homo sapiens
S38A
mutant enzyme with 74% of the in vivo activity of the wild-type enzyme
Homo sapiens
T124A
mutant enzyme with 91% of the in vivo activity of the wild-type enzyme
Homo sapiens
T126A
mutant enzyme with 4% of the in vivo activity of the wild-type enzyme
Homo sapiens
T126S
mutant enzyme still binds the inhibitor ifenprodil
Homo sapiens
V120A
mutant enzyme with 62% of the in vivo activity of the wild-type enzyme
Homo sapiens
V138A
mutant enzyme with 94% of the in vivo activity of the wild-type enzyme
Homo sapiens
V191A
mutant enzyme with 91% of the in vivo activity of the wild-type enzyme
Homo sapiens
V199A
mutant enzyme with 76% of the in vivo activity of the wild-type enzyme
Homo sapiens
V203A
mutant enzyme with 71% of the in vivo activity of the wild-type enzyme
Homo sapiens
V39A
mutant enzyme with 80% of the in vivo activity of the wild-type enzyme
Homo sapiens
V44A
mutant enzyme with 76% of the in vivo activity of the wild-type enzyme
Homo sapiens
V45A
mutant enzyme with 79% of the in vivo activity of the wild-type enzyme
Homo sapiens
V72A
mutant enzyme with 93% of the in vivo activity of the wild-type enzyme
Homo sapiens
V79A
mutant enzyme with 75% of the in vivo activity of the wild-type enzyme
Homo sapiens
V85A
mutant enzyme with 68% of the in vivo activity of the wild-type enzyme
Homo sapiens
W130A
mutant enzyme with 70% of the in vivo activity of the wild-type enzyme
Homo sapiens
W136A
mutant enzyme with 104% of the in vivo activity of the wild-type enzyme
Homo sapiens
W187A
mutant enzyme with 56% of the in vivo activity of the wild-type enzyme
Homo sapiens
W197A
mutant enzyme with 5% of the in vivo activity of the wild-type enzyme
Homo sapiens
W197F
mutant enzyme still binds the inhibitor ifenprodil
Homo sapiens
W48A
mutant enzyme with 87% of the in vivo activity of the wild-type enzyme
Homo sapiens
W69A
mutant enzyme with 17% of the in vivo activity of the wild-type enzyme
Homo sapiens
W83A
mutant enzyme with 97% of the in vivo activity of the wild-type enzyme
Homo sapiens
Y185A
mutant enzyme with 64% of the in vivo activity of the wild-type enzyme
Homo sapiens
Y189A
mutant enzyme with 32% of the in vivo activity of the wild-type enzyme
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ifenprodil
-
Homo sapiens
Other publictions for EC 5.3.3.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726836
Krojer
Steroidomimetic aminomethyl sp ...
Homo sapiens
Arch. Pharm.
347
108-122
2014
-
-
-
-
-
-
6
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
3
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727162
Koenig
Stereoselective synthesis of a ...
Saccharomyces cerevisiae, [Candida] glabrata, Yarrowia lipolytica, Homo sapiens
Bioorg. Med. Chem.
21
1925-1943
2013
-
-
-
-
-
-
64
-
-
-
-
8
-
4
-
-
-
-
-
-
-
-
8
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
64
-
-
-
-
-
8
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714753
Abate
1-Cyclohexyl-4-(4-arylcyclohex ...
Homo sapiens
ChemMedChem
6
73-80
2011
-
-
-
-
-
-
21
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
21
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690830
Rahier
Identification of essential am ...
Saccharomyces cerevisiae, Zea mays
Biochem. J.
414
247-259
2008
-
-
1
-
11
-
12
3
-
-
-
-
-
14
-
-
-
-
-
-
-
-
8
-
6
-
-
-
-
-
-
-
-
-
-
10
-
-
1
-
-
11
-
10
12
-
3
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693574
Berardi
Novel 4-(4-aryl)cyclohexyl-1-( ...
Homo sapiens
J. Med. Chem.
51
7523-7531
2008
-
-
-
-
-
-
28
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
4
-
-
-
-
-
-
-
-
28
-
-
-
-
-
-
-
-
-
-
28
28
-
-
-
1
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662248
Kedjouar
Molecular characterization of ...
Homo sapiens
J. Biol. Chem.
279
34048-34061
2004
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649741
Nes
Purification, characterization ...
Homo sapiens
Biochem. J.
367
587-599
2002
-
-
1
-
-
-
6
1
-
-
3
-
-
1
-
-
1
-
-
-
1
1
8
2
-
1
-
-
1
1
-
-
-
6
1
-
-
-
1
-
-
-
-
-
6
6
1
-
-
3
-
-
-
-
1
-
-
1
1
8
2
1
-
-
1
1
-
-
1
-
-
-
-
-
-
649702
Bae
Cholesterol biosynthesis from ...
Rattus norvegicus
Biochem. J.
353
689-699
2001
-
-
1
-
-
-
2
-
-
-
1
1
-
16
-
-
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
2
2
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649855
Moebius
Histidine77, glutamic acid81, ...
Homo sapiens
Biochemistry
38
1119-1127
1999
-
-
-
-
69
-
1
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
69
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653232
Cho
Cholesterol biosynthesis from ...
Rattus norvegicus
Mol. Cells
8
233-239
1998
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653279
Moebius
Pharmacological analysis of st ...
Saccharomyces cerevisiae, Mus musculus, Homo sapiens, Cavia porcellus
Mol. Pharmacol.
54
591-598
1998
-
-
-
-
-
-
85
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
51
-
18
-
-
-
-
-
-
-
18
85
51
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