Protein Variants | Comment | Organism |
---|---|---|
R39Q | titration with cis,cis-muconate shows negative cooperativity | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2E)-2-fluoro-2,4-pentadienoate | - |
Pseudomonas putida | |
(2E)-fluoromuconate | a dicarboxylate competitive inhibitor, shows substoichiometric binding to 3 +/- 1 sites per hexamer | Pseudomonas putida | |
(2Z)-2-fluoro-2,4-pentadienoate | - |
Pseudomonas putida | |
additional information | although 4-OT exhibits no structural asymmetry either by X-ray or by NMR, inactivation by two affinity labels shows half-site stoichiometry | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 4-OT exhibits hyperbolic kinetics and shows anticooperativity during catalysis | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-hexenedioate | the enzyme converts the unconjugated enone to the conjugated enone via a dienolic intermediate 2-hydroxymuconate, Pro1 serves as the general base, and both Arg11 and Arg39 function in substrate binding and catalysis in an otherwise hydrophobic active site. Anticooperativity during catalysis | Pseudomonas putida | 2-oxo-3-hexenedioate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | - |
Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
4-OT | - |
Pseudomonas putida |
4-oxalocrotonate tautomerase | - |
Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
(2E)-fluoromuconate | pH and temperature not specified in the publication | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
additional information | structure-function relationship and kinetic analysis, detailed overview | Pseudomonas putida |