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Literature summary for 5.3.1.9 extracted from

  • Somarowthu, S.; Brodkin, H.R.; DAquino, J.A.; Ringe, D.; Ondrechen, M.J.; Beuning, P.J.
    A tale of two isomerases: compact versus extended active sites in ketosteroid isomerase and phosphoglucose isomerase (2011), Biochemistry, 50, 9283-9295.
    View publication on PubMed
Search for more literature for 5.3.1.9

Protein Variants

Protein Variants Comment Organism
D511N the mutant shows 0.44% activity compared to the wild type enzyme Homo sapiens
E495Q the mutant shows 0.39% activity compared to the wild type enzyme Homo sapiens
H100L the mutant shows 0.16% activity compared to the wild type enzyme Homo sapiens
H389L inactive Homo sapiens
H396L the mutant shows 4.2% activity compared to the wild type enzyme Homo sapiens
K362A inactive Homo sapiens
N154Q the mutant shows 26% activity compared to the wild type enzyme Homo sapiens
N386A the mutant shows 36% activity compared to the wild type enzyme Homo sapiens
Q388A the mutant shows 5.3% activity compared to the wild type enzyme Homo sapiens
S185A the mutant shows 29% activity compared to the wild type enzyme Homo sapiens
Y274F the mutant shows 46% activity compared to the wild type enzyme Homo sapiens
Y341F the mutant shows 65% activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 D-glucose 6-phosphate mutant enzyme D511N, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
0.2 1 D-glucose 6-phosphate mutant enzyme H100L, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
0.23
-
 D-glucose 6-phosphate mutant enzyme E495Q, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
0.29
-
 D-glucose 6-phosphate mutant enzyme H396L, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
0.29
-
 D-glucose 6-phosphate wild type enzyme, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
0.3
-
 D-glucose 6-phosphate mutant enzyme Y274F, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
0.3
-
 D-glucose 6-phosphate mutant enzyme Y341F, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
0.5
-
 D-glucose 6-phosphate mutant enzyme Q388A, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
0.73
-
 D-glucose 6-phosphate mutant enzyme S185A, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
0.78
-
 D-glucose 6-phosphate mutant enzyme N386A, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
1.04
-
 D-glucose 6-phosphate mutant enzyme N154Q, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate Homo sapiens
-
 D-fructose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P06744
-
-

Purification (Commentary)

Purification (Comment) Organism
MonoQ column chromatography and SP ion-exchange column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate
-
 Homo sapiens D-fructose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PGI
-
 Homo sapiens
Phosphoglucose isomerase
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
54.8
-
 the melting temperature of the mutant is 54.8°C Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 D-glucose 6-phosphate kcat below 0.04 s-1,mutant enzyme K362A, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
0.04
-
 D-glucose 6-phosphate kcat below 0.04 s-1mutant enzyme H389L, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
0.6
-
 D-glucose 6-phosphate mutant enzyme H100L, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
1.3
-
 D-glucose 6-phosphate mutant enzyme D511N, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
1.6
-
 D-glucose 6-phosphate mutant enzyme E495Q, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
4 5 D-glucose 6-phosphate mutant enzyme Q388A, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
21
-
 D-glucose 6-phosphate mutant enzyme H396L, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
240
-
 D-glucose 6-phosphate mutant enzyme Y274F, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
340
-
 D-glucose 6-phosphate mutant enzyme Y341F, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
360
-
 D-glucose 6-phosphate mutant enzyme S185A, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
470
-
 D-glucose 6-phosphate mutant enzyme N154Q, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
470
-
 D-glucose 6-phosphate mutant enzyme N386A, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
500
-
 D-glucose 6-phosphate wild type enzyme, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens

General Information

General Information Comment Organism
malfunction enzyme deficiency leads to nonspherocytic hemolytic anemia Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7
-
 D-glucose 6-phosphate mutant enzyme H100L, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
6.7
-
 D-glucose 6-phosphate mutant enzyme E495Q, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
7.4
-
 D-glucose 6-phosphate mutant enzyme D511N, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
72
-
 D-glucose 6-phosphate mutant enzyme H396L, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
90
-
 D-glucose 6-phosphate mutant enzyme Q388A, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
450
-
 D-glucose 6-phosphate mutant enzyme N154Q, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
490
-
 D-glucose 6-phosphate mutant enzyme S185A, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
610
-
 D-glucose 6-phosphate mutant enzyme N386A, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
790
-
 D-glucose 6-phosphate mutant enzyme Y274F, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
1100
-
 D-glucose 6-phosphate mutant enzyme Y341F, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens
1700
-
 D-glucose 6-phosphate wild type enzyme, at 21°C in 20 mM HEPES buffer (pH 7.5) Homo sapiens